Mercury (II) ions are considered the most harmful heavy metal ion and their accumulation in the human body causes several diseases so need to be detected at trace levels. The use of nanozyme-based colorimetric detection is an acknowledged and innovative method to detect heavy metal ions in water bodies. In this work, we report CuMOF nanoparticles (CuMOF NPs) synthesized using hand grinding technique. The MOF nanoparticles were characterized by FTIR, TGA, SCXRD, PXRD, FESEM and TEM. The SCXRD confirmed paddle wheel type structure and MOF exhibits interpenetrated pcu topology with point symbol {4¹².6³}. BET analysis revealed a high surface area of 15.995 m²g^-1 and pore diameter of 3.42 nm for CuMOF NPs. The MOF NPs were found to show peroxidase-like activity. The CuMOF NPs employed as the catalyst, where 3,3′,5,5′-tetramethylbenzidine (TMB) was oxidised by Hg²⁺ and a bluish solution of oxidised TMB was obtained and recorded by UV–vis spectroscopy. The activity was determined with a detection limit of 0.55 μM, K_m of 0.0477 M, and V_max of 0.3125 M s⁻¹ for TMB, and K_m of 2.646 M and V_max of 0.126 M s⁻¹ for H₂O₂. The selectivity of the method was excellent and the mechanism was supported by XPS analysis. So, we provide a low-cost, eco-friendly approach for the detection of Hg²⁺ using CuMOF NPs.

汞 (II) 离子被认为是最有害的重金属离子，它们在人体内的积累会导致多种疾病，因此需要进行痕量检测。使用基于纳米酶的比色检测是一种公认​​的创新方法来检测水体中的重金属离子。在这项工作中，我们报告了使用手工研磨技术合成的 CuMOF 纳米粒子 (CuMOF NPs)。通过 FTIR、TGA、SCXRD、PXRD、FESEM 和 TEM 对 MOF 纳米粒子进行了表征。SCXRD 确认的桨轮式结构和 MOF 展示了带有点符号 {4 ¹² .6 ³ }的相互穿插的pcu拓扑结构。BET 分析表明表面积高达 15.995 m ² g ^-1CuMOF NPs 的孔径为 3.42 nm。发现 MOF NP 显示出类似过氧化物酶的活性。CuMOF NPs 用作催化剂，其中 3,3',5,5'-四甲基联苯胺 (TMB) 被 Hg ²⁺氧化，得到氧化 TMB 的蓝色溶液，并通过紫外-可见光谱进行记录。TMB的活性检测限为 0.55 μM，K _m为 0.0477 M，V _max为 0.3125 M s ^-1，H ₂ O ₂的K _m为 2.646 M，V _max为 0.126 M s ^-1. 该方法的选择性非常好，其机理得到了 XPS 分析的支持。因此，我们提供了一种使用 CuMOF NP检测 Hg ²⁺的低成本、环保方法。