长支链支链淀粉的产生对于控制淀粉功能具有重要意义。支链淀粉的长支链目前主要是通过支链淀粉酶对支链淀粉的延伸合成,但由于难以大规模生产支链淀粉酶,这种方法很难实现工业化。本研究旨在建立一种通过预热 (58–73 ℃)、普鲁兰酶消化和离心从糯玉米淀粉中分离出长支链支链淀粉的简便方法。结果表明,支链淀粉链长与淀粉的热稳定性呈正相关。由短支链支链淀粉包裹的结构在预热过程中会发生糊化,然后通过支链淀粉酶消化和离心从淀粉颗粒中去除,以收集富含长支链支链淀粉的热稳定结构。高温预热促进了淀粉糊化和普鲁兰酶消化,进而增加了所收集淀粉中长支链支链淀粉的含量。通过预热-普鲁兰酶消化-离心处理,淀粉中短支链支链淀粉的含量由24.04%显着降低至19.69%,收集到富含长支链支链淀粉的淀粉。研究结果证实,通过预热、普鲁兰酶消化和离心分离的组合处理,可以从淀粉颗粒中分离出长支链支链淀粉。高温预热促进了淀粉糊化和普鲁兰酶消化,进而增加了所收集淀粉中长支链支链淀粉的含量。通过预热-普鲁兰酶消化-离心处理,淀粉中短支链支链淀粉的含量由24.04%显着降低至19.69%,收集到富含长支链支链淀粉的淀粉。研究结果证实,通过预热、普鲁兰酶消化和离心分离的组合处理,可以从淀粉颗粒中分离出长支链支链淀粉。高温预热促进了淀粉糊化和普鲁兰酶消化,进而增加了所收集淀粉中长支链支链淀粉的含量。通过预热-普鲁兰酶消化-离心处理,淀粉中短支链支链淀粉的含量由24.04%显着降低至19.69%,收集到富含长支链支链淀粉的淀粉。研究结果证实,通过预热、普鲁兰酶消化和离心分离的组合处理,可以从淀粉颗粒中分离出长支链支链淀粉。淀粉的短支链支链淀粉含量从24.04%显着下降到19.69%,收集到富含长支链支链淀粉的淀粉。研究结果证实,通过预热、普鲁兰酶消化和离心分离的组合处理,可以从淀粉颗粒中分离出长支链支链淀粉。淀粉的短支链支链淀粉含量从24.04%显着下降到19.69%,收集到富含长支链支链淀粉的淀粉。研究结果证实,通过预热、普鲁兰酶消化和离心分离的组合处理,可以从淀粉颗粒中分离出长支链支链淀粉。
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A facile method for isolating long branch-chains of amylopectin from starch by preheating and pullulanase treatment
Production of long branch-chains of amylopectin was significant to conftrol starch functionalities. Long branch-chains of amylopectin were currently synthesized through amylopectin elongation by amylosucrase, but this methodology was hardly industrialized because of the difficulty in large-scale production of amylosucrase. This study aimed to establish a facile method to fractionate long branch-chains of amylopectin from waxy maize starch following preheating (58–73 ℃), pullulanase digestion, and centrifugation. Results showed that amylopectin chain length was positively correlated with starch thermostability. Structures packed by short branch-chains of amylopectin would be gelatinized during preheating, and then removed from starch granules by pullulanase digestion and centrifugation to collect thermostable structures rich in long branch-chains of amylopectin. The preheating at higher temperatures promoted starch gelatinization and pullulanase digestion, and in turn, increased the content of long branch-chains of amylopectin of collected starch. By the preheating-pullulanase digestion-centrifugation treatment, the content of short branch-chains of amylopectin of starch significantly decreased from 24.04% to 19.69% and starch rich in long branch-chains of amylopectin was collected. Findings verified that long branch-chains of amylopectin could be isolated from starch granules following the combination treatments of preheating, pullulanase digestion, and centrifugation.