通过静电纺丝组织衍生的脱细胞细胞外基质仿生肌腱用于肌腱组织工程,Journal of Applied Polymer Science

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通过静电纺丝组织衍生的脱细胞细胞外基质仿生肌腱用于肌腱组织工程
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2022-11-17 , DOI: 10.1002/app.53368
Aakanksha Ruhela ₁ , Akshay Bhatt ₂ , Subha Narayan Rath ₂ , Chandra Shekhar Sharma ₁

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肌腱损伤扰乱了活动性和稳定性之间的平衡，导致功能受损和残疾。在临床上，再生功能齐全的肌腱仍然具有挑战性。在这里，通过直接静电纺丝，我们使用聚己内酯 (PCL) 作为支撑聚合物来制造山羊肌腱衍生的细胞外基质 (tdECM) 纤维。我们观察到 tdECM 颗粒的掺入强烈影响支架的特性，例如润湿性、吸水能力和机械性能。与仅 PCL 的 122° 相比，PCL/tdECM 支架的接触角降至零，从而使支架完全亲水。通过添加 tdECM，吸水能力提高了 200%。使用傅里叶变换红外光谱 (FTIR) 和扫描电子显微镜 (SEM) 评估物理化学性质。电纺纤维模仿天然 ECM 结构，而 tdECM 可以为人类间充质干细胞粘附和分化提供生化线索。支架对人脐带来源的间充质干细胞 (hUMSC) 支架的细胞存活、增殖和排列产生积极影响。本研究展示了电纺 ECM/聚合物作为原位肌腱再生生物活性支架的潜力。以及沿着人类脐带来源的间充质干细胞 (hUMSC) 支架排列。本研究展示了电纺 ECM/聚合物作为原位肌腱再生生物活性支架的潜力。以及沿着人类脐带来源的间充质干细胞 (hUMSC) 支架排列。本研究展示了电纺 ECM/聚合物作为原位肌腱再生生物活性支架的潜力。

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Biomimicking tendon by electrospinning tissue-derived decellularized extracellular matrix for tendon tissue engineering

Tendon injuries disturb the equilibrium between mobility and stability, resulting in impaired functions and disabilities. Clinically, it is still challenging to regenerate fully functional tendons. Here, by direct electrospinning, we fabricate goat tendon-derived extracellular matrix (tdECM) fibers using polycaprolactone (PCL) as a supporting polymer. We observe that the incorporation of tdECM particles strongly influences the characteristics of the scaffold, such as wettability, water uptake ability, and mechanical properties. The contact angle of the PCL/tdECM scaffold decreases to zero as compared to 122° for only PCL, making the scaffold completely hydrophilic. The water uptake ability increases by 200% by adding tdECM. The Physicochemical properties are evaluated using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrospun fibers mimic the natural ECM structure, while tdECM can provide biochemical cues for the human mesenchymal stem cells to adhere to and differentiate. The scaffolds positively influence cell survival, proliferation, and alignment along the scaffolds of the human umbilical cord-derived mesenchymal stem cells (hUMSCs). This study demonstrates the potential of electrospun ECM/polymer as a bioactive scaffold for in situ tendon regeneration.

更新日期：2022-11-17

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