Glucose detection is vital in the food industry for safety and quality management. As a healthy ingredient, the flavor of honey is frequently impacted by the crystallization of glucose. Therefore, determining the glucose level of honey can offer precise reference data for the manufacture of honey. Various approaches have been tried, and the enzyme-based electrochemical analytical method is one of the most important and widely used strategies. However, there are still challenges for most electrochemical methods to achieve stable detection resistant to temperature variation due to the easy inactivation of the enzyme, the poor anti-interference capacity of the detection techniques and other influences from the external environment. Herein, a hydrogel-based electrochemical sensor is proposed to stably detect glucose even at wide ranges of temperatures via electrochemical impedance spectroscopic (EIS) measurement. The key factor for stable detection relies on the metal-organic framework nanoparticles' protective layer to guarantee the robustness of glucose oxidase (GOx), thereby achieving stable and specific detection for glucose. Moreover, a cascade reaction-induced hydrogel formation in a 3D structure can be used as an impedance readout, which not only amplifies but also further stabilizes the GOx-induced response. The prepared hydrogel-based electrochemical sensor showed a linear response to the glucose concentration in the range of 0.75–4 mg/mL. Furthermore, the biosensor has excellent anti-interference and temperature stability. High performance liquid chromatography analysis also validated the accuracy of this biosensor in detecting glucose in the honey sample.

葡萄糖检测对于食品行业的安全和质量管理至关重要。作为一种健康成分，蜂蜜的味道经常受到葡萄糖结晶的影响。因此，测定蜂蜜的葡萄糖含量可以为蜂蜜的生产提供准确的参考数据。已经尝试了各种方法，基于酶的电化学分析方法是最重要和广泛使用的策略之一。然而，由于酶的易失活、检测技术的抗干扰能力差以及外界环境的其他影响，大多数电化学方法在实现抗温度变化的稳定检测方面仍然存在挑战。在此，提出了一种基于水凝胶的电化学传感器，即使在很宽的温度范围内也能稳定检测葡萄糖通过电化学阻抗谱 (EIS) 测量。稳定检测的关键因素依赖于金属有机骨架纳米粒子的保护层来保证葡萄糖氧化酶（GOx）的稳健性，从而实现对葡萄糖的稳定和特异性检测。此外，3D 结构中级联反应诱导的水凝胶形成可用作阻抗读数，这不仅放大而且进一步稳定了 GOx 诱导的响应。制备的基于水凝胶的电化学传感器对葡萄糖浓度在 0.75–4 mg/mL 范围内表现出线性响应。此外，该生物传感器具有优异的抗干扰性和温度稳定性。高效液相色谱分析也验证了该生物传感器检测蜂蜜样品中葡萄糖的准确性。