As a result of large imaging depth, low background biofluorescence, facile operation, and high tempo-spatial resolution, the small-molecule near-infrared (NIR) fluorescence imaging technique has been one of the most popular tools for in situ monitoring of cellular oxidative stress (OS) processes. Nevertheless, the recent near infrared probes have low signal-to-noise ratio due to the small stokes shift. To this end, this work quinone-cyanine-7 (QCy7, ${\lambda }_{\mathit{max}}^{abs}$ = 582 nm, ε = 9329 M⁻¹ cm⁻¹ and ${\lambda }_{\mathit{max}}^{em}$ = 719 nm, Ф = 0.12) with large Stokes shift (137 nm) was used as a fluorophore, and 1-methylindoline-2,3-dione was used as the “Turn-ON” cyanine NIR Peroxynitrite (ONOO⁻) fluorescent probe HJ-ONOO-P3 (with 41-fold fluorescence enhancement with ONOO⁻ concentration in the range of 0–35 μM). The indole quaternary ammonium heterocycle with a positive charge itself could be used as the targeted group for mitochondria. This probe exhibited a rapid response (5 min complete response) and high selectivity and sensitivity to ONOO⁻ (detection limit as low as 25.4 nM). Meanwhile, this probe could achieve the task of in-situ imaging of OS during cellular oxygen-glucose deprivation/reperfusion (OGD/R) and middle cerebral artery occlusion (MCAO) in mice. The results demonstrated that the mitochondria-targeted NIR probe with a large Stokes shift possess great potential in studies of stroke and the induced cellular OS.

由于成像深度大、背景生物荧光低、操作简便、时空分辨率高，小分子近红外 (NIR) 荧光成像技术已成为细胞氧化原位监测最流行的工具之一。压力（OS）过程。然而，最近的近红外探测器由于斯托克斯位移小而具有低信噪比。为此，本工作 quinone-cyanine-7 (QCy7,${\lambda }_{\mathit{最大限度}}^{\mathrm{一个}bs}$ = 582 nm，ε = 9329 M ^-1  cm ^-1和${\lambda }_{\mathit{最大限度}}^{e米}$ = 719 nm, Ф = 0.12) 具有大斯托克斯位移 (137 nm) 用作荧光团，1-甲基二氢吲哚-2,3-二酮用作“开启”花青 NIR 过氧亚硝酸盐 (ONOO ^- ) 荧光探针HJ-ONOO-P3（具有 41 倍荧光增强，ONOO ^-浓度在 0-35 μM 范围内）。本身带正电荷的吲哚季铵杂环可作为线粒体的靶基。该探针表现出快速响应（5 分钟完全响应）以及对 ONOO 的高选择性和灵敏度^-（检测限低至 25.4 nM）。同时，该探针可以实现小鼠细胞氧葡萄糖剥夺/再灌注（OGD/R）和大脑中动脉闭塞（MCAO）过程中OS的原位成像任务。结果表明，具有大斯托克斯位移的线粒体靶向近红外探针在卒中和诱导的细胞 OS 研究中具有巨大潜力。