Chemical investigation of the plant Gymnema latifolium led to the isolation of seven undescribed 23-glycosyl oleanane triterpenoids, gymlatinosides GLF1-GLF7, and two known compounds, gymnemosides D and E. The structures of the isolated compounds were elucidated using diverse spectroscopic methods. The extract of G. latifolium and all isolated compounds significantly enhanced 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) uptake into 3T3-L1 adipocytes at 20 μM. Among them, gymlatinosides GLF2 and gymlatinosides GLF4 showed particularly potent stimulatory effects on glucose uptake in a dose-dependent manner. Further investigation revealed that gymlatinosides GLF2 at 20 μM upregulated the expression of phosphorylated AMPK (p-AMPK). The results suggested that gymlatinosides GLF2 may enhance glucose uptake via regulating the AMPK signaling pathway.

对匙羹藤植物的化学研究导致分离出七种未描述的 23-糖基齐墩果烷三萜类化合物、gymlatinosides GLF1-GLF7 和两种已知的化合物、gymnemosides D 和 E。使用多种光谱方法阐明了分离化合物的结构。G. latifolium的提取物和所有分离的化合物显着增强了 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) 对 3T3 的吸收-L1 脂肪细胞，浓度为 20 μM。其中，gymlatinosides GLF2 和 gymlatinosides GLF4 以剂量依赖的方式显示出对葡萄糖摄取特别有效的刺激作用。进一步研究表明，20 μM 的 gymlatinosides GLF2 上调磷酸化 AMPK 的表达（p-AMPK）。结果表明，gymlatinosides GLF2 可能通过调节 AMPK 信号通路增强葡萄糖摄取。