Epigallocatechin gallate and tetrahydrocurcumin are aminated as colonic metabolites, preserving their bioactivities and improving their capabilities. We compared the bioactivities of unaminated (CUR) and aminated (AC) curcumin in inflammatory colitis-associated tumorigenesis. The anti-inflammatory and anticancer capabilities of CUR and AC were evaluated using RAW264.7 and HT29 cell lines, respectively. An azoxymethane/dextran sodium sulfate-induced colitis-associated carcinogenesis mouse model was used with CUR and two-dose AC interventions. AC had a greater anti-inflammatory effect but a similar anticancer effect as CUR in vitro. CUR and low-dose AC (LAC) significantly preserved colon length and reduced tumor number in vivo. Both CUR and LAC inhibited activation of the protein kinase B (AKT)/nuclear factor kappa B (NF-κB) signaling pathway, its downstream cytokines, and the interleukin (IL)-6/signal transducer and activator of transcription 3 (STAT3)/c-myelocytomatosis oncogene (c-MYC) pathway. However, only LAC significantly preserved E-cadherin, reduced N-cadherin, and facilitated beneficial gut microbial growth, including Akkermansia and Bacteroides, potentially explaining AC’s better ameliorative effect at low than high doses.

中文翻译：

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胺化作用可能会增强姜黄素对 IL-6/Stat3/c-Myc 通路抑制和结肠炎相关肿瘤发生中肠道微生物调节的改善作用

表没食子儿茶素没食子酸酯和四氢姜黄素被胺化为结肠代谢物，保留了它们的生物活性并提高了它们的能力。我们比较了未胺化 (CUR) 和胺化 (AC) 姜黄素在炎症性结肠炎相关肿瘤发生中的生物活性。分别使用 RAW264.7 和 HT29 细胞系评估了 CUR 和 AC 的抗炎和抗癌能力。氧化偶氮甲烷/葡聚糖硫酸钠诱导的结肠炎相关致癌小鼠模型与 CUR 和两剂量 AC 干预一起使用。AC 具有更强的抗炎作用，但在体外与 CUR 具有相似的抗癌作用。CUR 和低剂量 AC (LAC) 显着保留了结肠长度并减少了体内肿瘤数量。CUR 和 LAC 均抑制蛋白激酶 B (AKT)/核因子 kappa B (NF-κB) 信号通路的激活，其下游细胞因子，以及白介素 (IL)-6/信号转导和转录激活因子 3 (STAT3)/c-myelocytomatosis 癌基因 (c-MYC) 通路。然而，只有 LAC 显着保留了 E-钙粘蛋白，减少了 N-钙粘蛋白，并促进了有益的肠道微生物生长，包括Akkermansia和Bacteroides，可能解释了 AC 在低剂量下比在高剂量下具有更好的改善效果。