聚多巴胺辅助肝素锚增强聚氨酯的亲水性、血液相容性和生物相容性,Journal of Applied Polymer Science

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聚多巴胺辅助肝素锚增强聚氨酯的亲水性、血液相容性和生物相容性
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2022-11-11 , DOI: 10.1002/app.53352
Heng Li ₁ , Yu Guo ₂ , Buyun Ma ₃ , Yunzhu Qian ₂ , Wentao Sun ₃ , Xuefeng Zhou ₁

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表面肝素化是解决小口径人工血管材料聚氨酯（PU）内皮化率低、抗凝性差、血液相容性差的有效解决方案。在这里，探讨了聚多巴胺 (PDA) 和聚丙烯酸 (PAA) 作为交联剂对表面肝素化的影响。与通过丙烯酸接枝的肝素化 PU 膜 (PU/PAA-Hep) 相比，通过多巴胺接枝肝素 (Hep) 的 PU 膜 (PU/PDA-Hep) 显示出更好的亲水性和稳定性。X射线光电子能谱结果表明，当PDA作为交联剂时，肝素成功接枝到PU表面，接枝效率高。接枝的肝素聚集形成纳米颗粒，增加了PU膜的表面粗糙度。肝素化膜对牛血清白蛋白和纤维蛋白具有良好的抗粘附性。此外，通过血小板粘附试验未发现肝素化 PU 上的活化血小板或离析物，这意味着肝素固定化表面具有良好的血液相容性。此外，体外细胞相容性评估表明，PU/PDA-Hep 显着改善了 L929 细胞的增殖，优于 PU/AA-Hep。这些结果表明，PDA 辅助表面肝素化是提高 PU 小直径血管材料抗凝和生物相容性的有效方法，可以扩展到其他植入材料。这意味着肝素固定化表面具有良好的血液相容性。此外，体外细胞相容性评估表明，PU/PDA-Hep 显着改善了 L929 细胞的增殖，优于 PU/AA-Hep。这些结果表明，PDA 辅助表面肝素化是提高 PU 小直径血管材料抗凝和生物相容性的有效方法，可以扩展到其他植入材料。这意味着肝素固定化表面具有良好的血液相容性。此外，体外细胞相容性评估表明，PU/PDA-Hep 显着改善了 L929 细胞的增殖，优于 PU/AA-Hep。这些结果表明，PDA 辅助表面肝素化是提高 PU 小直径血管材料抗凝和生物相容性的有效方法，可以扩展到其他植入材料。

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The polydopamine-assisted heparin anchor enhances the hydrophilicity, hemocompatibility, and biocompatibility of polyurethane

Surface heparinization is an effective solution to resolve low endothelialization, poor anticoagulation, and hemocompatibility of polyurethane (PU) used as materials of small-diameter vascular grafts. Here, the effects of polydopamine (PDA) and poly (acrylic acid) (PAA) as crosslinking agents on the surface heparinization were explored. The PU membranes grafted with heparin (Hep) via dopamine (PU/PDA-Hep) showed better hydrophilicity and stability, compared to heparinized PU membranes via acrylic acid (PU/PAA-Hep). The results of X-ray photoelectron spectroscopy demonstrated that heparin was successfully grafted onto the PU surface and the grafting efficiency was high when PDA as a cross-linking agent. The grafted heparin aggregated and formed nanoparticles, and increased the surface roughness of PU membranes. The heparinized membranes demonstrated good anti-adhesion of bovine serum albumin and fibrin protein. In addition, no activated platelets or educts on heparinized PU were found by platelet adhesion tests, implying that heparin-immobilized surfaces had good hemocompatibility. Moreover, the in vitro cytocompatibility assessment showed that the PU/PDA-Hep significantly improved the proliferation of L929 cells and was superior to PU/AA-Hep. These results demonstrated that PDA-assisted surface heparinization was an effective method to improve the anticoagulant and biocompatibility of PU small-diameter vascular materials and could be extended to other implantable materials.

更新日期：2022-11-11

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