Planta ( IF 3.6 ) Pub Date : 2022-11-11 , DOI: 10.1007/s00425-022-04018-7 Anas Abuzeineh 1 , Grégory Vert 2 , Enric Zelazny 1
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Main conclusion
IRT1 intracellular dynamics and function are finely controlled through protein–protein interactions.
Abstract
In plants, iron uptake from the soil is tightly regulated to allow optimal growth and development. Iron acquisition in Arabidopsis root epidermal cells requires the IRT1 transporter, which also mediates the entry of non-iron metals. In this mini-review, we describe how protein-protein interactions regulate IRT1 intracellular dynamics and IRT1-mediated metal uptake to maintain iron homeostasis. Recent interactomic data provided interesting clues on IRT1 secretion and the putative involvement of COPI- and COPII-mediated pathways. Once delivered to the plasma membrane, IRT1 can interact with other components of the iron uptake machinery to form an iron acquisition complex that likely optimizes iron entrance in root epidermal cells. Then, IRT1 may be internalized from the plasma membrane. In the past decade, IRT1 endocytosis emerged as an essential mechanism to control IRT1 subcellular localization and thus to tune iron uptake. Interestingly, IRT1 endocytosis and degradation are regulated by its non-iron metal substrates in an ubiquitin-dependent manner, which requires a set of interacting-proteins including kinases, E3 ubiquitin ligases and ESCRT complex subunits. This mechanism is essential to avoid non-iron metal overload in Arabidopsis when the iron is scarce.
Graphical Abstract
中文翻译：
拟南芥 IRT1 铁转运蛋白的诞生、生死：密友和敌人的角色
主要结论
IRT1 细胞内动力学和功能通过蛋白质-蛋白质相互作用得到精细控制。
抽象的
在植物中，从土壤中吸收铁元素受到严格调控，以实现最佳生长和发育。拟南芥中的铁获取根表皮细胞需要 IRT1 转运蛋白，它还介导非铁金属的进入。在这篇小型综述中，我们描述了蛋白质-蛋白质相互作用如何调节 IRT1 细胞内动力学和 IRT1 介导的金属摄取以维持铁稳态。最近的相互作用组学数据提供了关于 IRT1 分泌和 COPI 和 COPII 介导途径的推定参与的有趣线索。一旦传送到质膜，IRT1 可以与铁摄取机制的其他组件相互作用，形成铁获取复合物，可能优化根表皮细胞中的铁进入。然后，IRT1 可能从质膜内化。在过去的十年中，IRT1 内吞作用成为控制 IRT1 亚细胞定位从而调节铁摄取的重要机制。有趣的是，IRT1 的内吞作用和降解受其非铁金属底物以泛素依赖性方式调节，这需要一组相互作用蛋白，包括激酶、E3 泛素连接酶和 ESCRT 复合亚基。这种机制对于避免非铁金属过载至关重要缺铁时的拟南芥。
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