Sensors based on the disappear of unique optical and magnetic signals of luminescent radicals caused by the conversion from open-shell into closed-shell molecules have not been realized. Here, we report a multiple-channel Cysteine (Cys) probe by a luminescent tris(2,4,6-trichloro-3-nitrophenyl) methyl radical (TTM^•-3NO₂) with good selectivity and high sensitivity. Cys has been confirmed to react with TTM^•-3NO₂ and generate closed-shell molecules, which causes the changes of fluorescence, colorimetry and electron paramagnetic resonance (EPR) of the radical. Interestingly, based on the above multiple-channel signal, we expanded the application of the radical sensor in RGB pattern, simulated clinical laboratory and fluorescent imaging. Our results not only display a promising Cys probe but also show a novel route for the application of organic radicals in sensing.

基于由开壳层分子转化为闭壳层分子引起的发光自由基的独特光磁信号消失的传感器尚未实现。在这里，我们报告了一种由发光的三（2,4,6-三氯-3-硝基苯基）甲基自由基 ( TTM ^• -3NO ₂ ) 组成的多通道半胱氨酸 (Cys) 探针，具有良好的选择性和高灵敏度。Cys 已被证实与TTM ^• -3NO _{2发生反应}并生成闭壳分子，引起自由基的荧光、比色和电子顺磁共振（EPR）的变化。有趣的是，基于上述多通道信号，我们扩展了自由基传感器在RGB模式、模拟临床实验室和荧光成像中的应用。我们的结果不仅展示了一种很有前途的 Cys 探针，而且还展示了有机自由基在传感中应用的新途径。