小鼠胰岛素生长因子样 (IGFL) 和人 IGFL1 蛋白在炎症性皮肤病中被诱导，并与新的肿瘤坏死因子受体家族成员 IGFLR1 结合。,Journal of Biological Chemistry

当前位置： X-MOL 学术 › J. Biol. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

小鼠胰岛素生长因子样 (IGFL) 和人 IGFL1 蛋白在炎症性皮肤病中被诱导，并与新的肿瘤坏死因子受体家族成员 IGFLR1 结合。
Journal of Biological Chemistry ( IF 4.0 ) Pub Date : 2011 May 27 , DOI: 10.1074/jbc.m111.224626
Adrian A Lobito ₁ , Sree R Ramani , Irene Tom , J Fernando Bazan , Elizabeth Luis , Wayne J Fairbrother , Wenjun Ouyang , Lino C Gonzalez

Affiliation

银屑病是一种人类皮肤病，其特征是表皮过度增殖和多种白细胞群的浸润。在对小鼠 (mIGFL) 中的一种新型胰岛素生长因子 (IGF) 样 (IGFL) 基因进行表征时，我们发现该基因的转录本在皮肤中表达最高，在皮肤创伤和银屑病样炎症模型中的表达增强。人类可能的功能直系同源物 IGFL1 在银屑病皮肤样本中被独特且显着地诱导。体外 IGFL1 表达在培养的原代角质形成细胞中被肿瘤坏死因子 α 上调，但不被其他银屑病相关细胞因子刺激。最后，使用分泌的跨膜蛋白文库，我们发现了人 IGFL1 和 mIGFL 与 TMEM149 胞外域之间的高亲和力相互作用。TMEM149（此处重命名为 IGFLR1）是一种未表征的基因，其结构与肿瘤坏死因子受体家族相似。我们的研究表明 IGFLR1 主要在小鼠 T 细胞表面表达。mIGFL 和 IGFLR1 受体之间的联系表明 mIGFL 可能会影响炎症性皮肤病中的 T 细胞生物学。

"点击查看英文标题和摘要"

Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1.

Psoriasis is a human skin condition characterized by epidermal hyperproliferation and infiltration of multiple leukocyte populations. In characterizing a novel insulin growth factor (IGF)-like (IGFL) gene in mice (mIGFL), we found transcripts of this gene to be most highly expressed in skin with enhanced expression in models of skin wounding and psoriatic-like inflammation. A possible functional ortholog in humans, IGFL1, was uniquely and significantly induced in psoriatic skin samples. In vitro IGFL1 expression was up-regulated in cultured primary keratinocytes stimulated with tumor necrosis factor alpha but not by other psoriasis-associated cytokines. Finally, using a secreted and transmembrane protein library, we discovered high affinity interactions between human IGFL1 and mIGFL and the TMEM149 ectodomain. TMEM149 (renamed here as IGFLR1) is an uncharacterized gene with structural similarity to the tumor necrosis factor receptor family. Our studies demonstrate that IGFLR1 is expressed primarily on the surface of mouse T cells. The connection between mIGFL and IGFLR1 receptor suggests mIGFL may influence T cell biology within inflammatory skin conditions.

更新日期：2017-01-31

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南