Pm36 的精细定位和候选基因分析，一种野生二粒小麦来源的硬粒小麦白粉病抗性位点,International Journal of Molecular Sciences

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Pm36 的精细定位和候选基因分析，一种野生二粒小麦来源的硬粒小麦白粉病抗性位点
International Journal of Molecular Sciences ( IF 4.9 ) Pub Date : 2022-11-07 , DOI: 10.3390/ijms232113659
Domenica Nigro ₁ , Antonio Blanco ₁ , Luciana Piarulli ₁ , Massimo Antonio Signorile ₁ , Pasqualina Colasuonno ₁ , Emanuela Blanco ₂ , Rosanna Simeone ₁

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白粉病 (PM) 是世界范围内栽培谷物的一种具有重要经济意义的叶面病害。种植抗病品种被认为是最有效、可持续和经济的疾病管理策略。本研究的目的是精细定位含有野生二粒小麦 PM 抗性基因座Pm36的染色体区域，并通过利用改良的四倍体小麦基因组资源来鉴定候选基因。使用 SNP 25K 芯片阵列对一组硬粒小麦回交近交系 (BIL) 进行基因分型，并比较抗 PM 和易感品系，定义了一个 1.5 cM 区域（物理间隔为 1.08 Mb），其中包含 Pm36。_{F 2:3}构建的遗传图谱通过杂交 PM 抗性品系 5BIL-42 和硬粒小麦亲本 Latino 获得的后代，Pm36和 SNP 标记 IWB22904 之间的遗传距离限制为 0.3 cM（物理距离 0.515 Mb）。包括Pm36在内的标记区间在四倍体小麦收集中的分布表明阳性等位基因主要存在于驯化和野生二粒小麦 Triticum turgidum spp。双球菌和 ssp。双球菌。在Pm36中鉴定出 10 个高可信度的蛋白质编码基因二粒小麦、硬粒小麦和面包小麦参考基因组的区域，而三个添加的基因在二粒小麦基因组中显示没有同源性。紧密连锁的标记可用于小麦育种计划中的标记辅助选择，并作为Pm36图谱克隆的起点。

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Fine Mapping and Candidate Gene Analysis of Pm36, a Wild Emmer-Derived Powdery Mildew Resistance Locus in Durum Wheat

Powdery mildew (PM) is an economically important foliar disease of cultivated cereals worldwide. The cultivation of disease-resistant varieties is considered the most efficient, sustainable and economical strategy for disease management. The objectives of the current study were to fine map the chromosomal region harboring the wild emmer PM resistance locus Pm36 and to identify candidate genes by exploiting the improved tetraploid wheat genomic resources. A set of backcross inbred lines (BILs) of durum wheat were genotyped with the SNP 25K chip array and comparison of the PM-resistant and susceptible lines defined a 1.5 cM region (physical interval of 1.08 Mb) harboring Pm36. The genetic map constructed with F_2:3 progenies derived by crossing the PM resistant line 5BIL-42 and the durum parent Latino, restricted to 0.3 cM the genetic distance between Pm36 and the SNP marker IWB22904 (physical distance 0.515 Mb). The distribution of the marker interval including Pm36 in a tetraploid wheat collection indicated that the positive allele was largely present in the domesticated and wild emmer Triticum turgidum spp. dicoccum and ssp. dicoccoides. Ten high-confidence protein coding genes were identified in the Pm36 region of the emmer, durum and bread wheat reference genomes, while three added genes showed no homologous in the emmer genome. The tightly linked markers can be used for marker-assisted selection in wheat breeding programs, and as starting point for the Pm36 map-based cloning.

更新日期：2022-11-07

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