沙田柚 (Citrus grandis var. Shatinyu Hort) 叶的转录组和代谢组分析提供了对赤霉素诱导基因 CcGASA4 过表达的见解,Frontiers in Plant Science

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沙田柚 (Citrus grandis var. Shatinyu Hort) 叶的转录组和代谢组分析提供了对赤霉素诱导基因 CcGASA4 过表达的见解
Frontiers in Plant Science ( IF 4.1 ) Pub Date : 2022-11-03 , DOI: 10.3389/fpls.2022.1022961
Tianli Wu _{1,

2} , Kaidong Liu ₁ , Min Chen ₂ , Bo Jiang ₂ , Qijing Gong ₁ , Yun Zhong _{2,

3,

4}

Affiliation

赤霉酸 (GA) 刺激拟南芥(GASA) 基因家族对植物具有高度特异性，在植物生长发育中起着至关重要的作用。CcGASA4是柑橘类植物中 GASA 基因家族的成员；然而，目前对其在柑橘中的功能的了解是有限的。我们使用 CcGASA4 过表达转基因柑橘 (OEGA) 和对照 (CON) 植物来研究CcGASA4在沙田柚。RNA 测序 (RNA-seq) 分析显示 3,522 个基因，包括 1,578 个上调基因和 1,944 个下调基因，在 CON 与 OEGA 组中显着差异表达。基因本体富集分析表明，178 个差异表达基因 (DEG) 与花有关。Kyoto Encyclopedia of Genes and Genomes（KEGG）富集分析表明，DEGs富集在134条通路中，包括“植物-病原体相互作用”、“MAPK信号通路-植物”、“苯丙烷生物合成”、“植物激素信号转导”、 “苯丙氨酸、酪氨酸和色氨酸的生物合成”，以及“类黄酮和黄酮醇的生物合成”。最显着富集的途径是“植物-病原体相互作用”，其中 203 个 DEGs 被富集（126 个 DEGs 被上调，78 个被下调）。代谢组分析表明，在 OEGA 和 CON 样品中检测到 644 种代谢物，包括 294 种差异积累的代谢物（DAM；与 CON 相比，OEGA 中 83 种上调和 211 种下调）。代谢途径分析表明，这些DAMs主要参与次级代谢产物的代谢途径，如苯丙素、苯丙氨酸、黄酮和黄酮醇的生物合成。十三种类黄酮和异黄酮被鉴定为 OEGA 和 CON 中的 DAM。我们还发现了 25 种 OEGA 特异性累积代谢物，并发现了 10 种与抗病性相关。包括 294 种差异积累的代谢物（DAM；与 CON 相比，OEGA 中 83 种上调和 211 种下调）。代谢途径分析表明，这些DAMs主要参与次级代谢产物的代谢途径，如苯丙素、苯丙氨酸、黄酮和黄酮醇的生物合成。十三种类黄酮和异黄酮被鉴定为 OEGA 和 CON 中的 DAM。我们还发现了 25 种 OEGA 特异性累积代谢物，并发现了 10 种与抗病性相关。包括 294 种差异积累的代谢物（DAM；与 CON 相比，OEGA 中 83 种上调和 211 种下调）。代谢途径分析表明，这些DAMs主要参与次级代谢产物的代谢途径，如苯丙素、苯丙氨酸、黄酮和黄酮醇的生物合成。十三种类黄酮和异黄酮被鉴定为 OEGA 和 CON 中的 DAM。我们还发现了 25 种 OEGA 特异性累积代谢物，并发现了 10 种与抗病性相关。十三种类黄酮和异黄酮被鉴定为 OEGA 和 CON 中的 DAM。我们还发现了 25 种 OEGA 特异性累积代谢物，并发现了 10 种与抗病性相关。十三种类黄酮和异黄酮被鉴定为 OEGA 和 CON 中的 DAM。我们还发现了 25 种 OEGA 特异性累积代谢物，并发现了 10 种与抗病性相关。CcGASA4因此可能在激活MAPK信号转导通路和抗病基因的表达，抑制生长素和乙烯相关基因的表达，以及激活或抑制油菜素类固醇生物合成和脱落酸相关基因的表达方面发挥功能性作用。CcGASA4还可能在调节类黄酮、异黄酮、氨基酸、嘌呤和酚类化合物的组成和丰度方面发挥作用。这项研究提供了对分子作用机制的新见解CcGASA4在柑橘类植物中。

"点击查看英文标题和摘要"

Transcriptome and metabolome analyses of Shatian pomelo (Citrus grandis var. Shatinyu Hort) leaves provide insights into the overexpression of the gibberellin-induced gene CcGASA4

The gibberellic acid (GA)-stimulated Arabidopsis (GASA) gene family is highly specific to plants and plays crucial roles in plant growth and development. CcGASA4 is a member of the GASA gene family in citrus plants; however, the current understanding of its function in citrus is limited. We used CcGASA4-overexpression transgenic citrus (OEGA) and control (CON) plants to study the role of CcGASA4 in Shatian pomelo. The RNA sequencing (RNA-seq) analysis showed that 3,522 genes, including 1,578 upregulated and 1,944 downregulated genes, were significantly differentially expressed in the CON versus OEGA groups. The Gene Ontology enrichment analysis showed that 178 of the differentially-expressed genes (DEGs) were associated with flowers. A Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the DEGs were enriched in 134 pathways, including “plant–pathogen interaction”, “MAPK signaling pathway-plant”, “phenylpropane biosynthesis”, “plant hormone signal transduction”, “phenylalanine, tyrosine and tryptophan biosynthesis”, and “flavonoid and flavonol biosynthesis”. The most significantly-enriched pathway was “plant–pathogen interaction”, in which 203 DEGs were enriched (126 DEGs were upregulated and 78 were downregulated). The metabolome analysis showed that 644 metabolites were detected in the OEGA and CON samples, including 294 differentially-accumulated metabolites (DAMs; 83 upregulated versus 211 downregulated in OEGA compared to CON). The metabolic pathway analysis showed that these DAMs were mainly involved in the metabolic pathways of secondary metabolites, such as phenylpropanoids, phenylalanine, flavone, and flavonol biosynthesis. Thirteen flavonoids and isoflavones were identified as DAMs in OEGA and CON. We also discovered 25 OEGA-specific accumulated metabolites and found 10 that were associated with disease resistance. CcGASA4 may therefore play a functional role in activating the expression of MAPK signaling transduction pathway and disease resistance genes, inhibiting the expression of auxin- and ethylene-related genes, and activating or inhibiting the expression of brassinosteroid biosynthesis- and abscisic acid-related genes. CcGASA4 may also play a role in regulating the composition and abundance of flavonoids, isoflavones, amino acids, purines, and phenolic compounds. This study provides new insights into the molecular mechanisms of action of CcGASA4 in citrus plants.

更新日期：2022-11-03

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