Fresh-market tomato is one of the most important vegetables in the United States (US), and novel, cost-effective labor solutions are an important issue in current fresh-market tomato production. The shortened stem driven by the brachytic (br) and the jointless pedicel driven by the jointless2 (j2) are two important traits that would contribute to knowledge-based breeding; based on these traits, an appropriate plant architecture can be designed for ground cultivation systems that will ultimately enable mechanical harvesting. We aimed to evaluate the effect of CRISPR-Cas9-driven shortened plant architecture and jointless pedicel on the yield and horticultural performance of fresh-market tomatoes using a ground cultivation system in an open field. We conducted field trials during three successive seasons in 2020 and 2021 using the fresh-market tomato variety Fla. 8059 (a non-brachytic jointed pedicel tomato; BR/BR:J2/J2; wild-type) and its three different CRISPR-Cas9-driven mutants, a brachytic jointed pedicel Fla. 8059 (br/br:J2/J2), a non-brachytic jointless pedicel Fla. 8059 (BR/BR:j2/j2), and a brachytic jointless pedicel Fla. 8059 (br/br:j2/j2). Field evaluations confirmed that the total yield of the mutants was not significantly different from the yield of the wild-type. However, there was a significant association between the high extra-large-sized fruit yield of the br mutant and the j2 mutation. Field evaluations also revealed a clear positive impact of the br mutation on the high proportion of fruits laying on the raised plastic bed. Our results contribute to the optimization of fresh-market tomato architecture for developing shortened jointless pedicel varieties and the adoption of low input management practices.

新鲜市场番茄是美国 (US) 最重要的蔬菜之一，新颖、具有成本效益的劳动力解决方案是当前新鲜市场番茄生产中的一个重要问题。由短枝( br )驱动的缩短的茎和由无节 2 ( j2驱动的无节花梗) 是有助于基于知识的育种的两个重要特征；基于这些特征，可以为地面耕作系统设计合适的植物结构，最终实现机械收割。我们的目的是评估 CRISPR-Cas9 驱动的缩短植物结构和无接缝花梗对使用露天地面栽培系统的新鲜市场番茄的产量和园艺性能的影响。我们在 2020 年和 2021 年连续三个季节使用新鲜市场番茄品种 Fla. 8059（一种非短枝有节番茄；BR/BR：J2/J2；野生型）及其三种不同的 CRISPR-Cas9进行了田间试验驱动突变体，一个短节的有节花梗 Fla. 8059 ( br/br : J2/J2)、非短枝无节花梗 Fla. 8059 ( BR/BR : j2/j2 ) 和短枝无节花梗 Fla. 8059 ( br/br : j2/j2 )。田间评估证实突变体的总产量与野生型的产量没有显着差异。然而， br突变体的超大型果实产量与j2突变体之间存在显着关联。现场评估还显示了br的明显积极影响高比例的水果放在凸起的塑料床上的突变。我们的结果有助于优化新鲜市场番茄结构，以开发缩短的无接柄品种和采用低投入管理实践。