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“Three-in-one” Zr-MOF Multifunctional Carrier-mediated Fluorescent and Colorimetric Dual-signal Readout Biosensing Platform to Enhance Analytical Performance
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2022-11-01 , DOI: 10.1021/acsami.2c16267
Liangqiong Ren 1, 2 , Feng Hong 1 , Lingwen Zeng 3 , Yiping Chen 1, 2, 4
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2022-11-01 , DOI: 10.1021/acsami.2c16267
Liangqiong Ren 1, 2 , Feng Hong 1 , Lingwen Zeng 3 , Yiping Chen 1, 2, 4
Affiliation
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To address the urgent demand for sensitive and stable detection applications, significant efforts have been made in the development of dual-signal readout assays for precise target detection and timely health risk control. Here, a new nanomaterial, Pt@PCN-224-HRP-initiator DNA (PP-HRP-iDNA), was exploited to construct a dual-signal readout biosensing platform. Zr-MOF (PCN-224) was loaded with as many Pt nanoparticles (NPs) and as much horseradish peroxidase (HRP) as possible to enhance the brightness of the colorimetric signal recognizable to the naked eye while also acting as a gatekeeper to protect the enzyme activity and ensuring the stability of the assay process. Moreover, the Pt NPs and HRP displayed a synergistic catalytic effect, which promoted the sensitivity of detection. Further, the formation of the Zr–O–P bond eliminated the instability of the interactions between PCN-224 and iDNA in a controllable manner. After the immunoreaction, iDNA stimulated a hybridization chain reaction, resulting in a significant reduction of the fluorescent DNA in the supernatant and a fluorescent signal change. Subsequently, the PP-HRP-iDNA probe implemented UV-light response (450 nm) where 3,3′,5,5′-tetramethylbenzidine was used as a substrate for the colorimetric signal readout. By virtue of the nanomaterial-modulated transduction mechanism and the antigen–antibody interactions, this dual-signal biosensor displays high sensitivity, with a limit of detection of 0.65 pg/mL for aflatoxin B1 and 4 CFU/mL for Salmonella enteritidis, suggesting the detection potential of the biosensing platform for analyzing various targets.
中文翻译：
“三合一”Zr-MOF 多功能载体介导的荧光和比色双信号读出生物传感平台可提高分析性能
为了满足对灵敏和稳定的检测应用的迫切需求，已经在开发用于精确目标检测和及时健康风险控制的双信号读出检测方面做出了重大努力。在这里，一种新的纳米材料 Pt@PCN-224-HRP-initiator DNA (PP-HRP-iDNA) 被用于构建双信号读出生物传感平台。Zr-MOF (PCN-224) 装载尽可能多的 Pt 纳米粒子 (NPs) 和辣根过氧化物酶 (HRP)，以增强肉眼可识别的比色信号的亮度，同时还充当守门人以保护酶活性并确保测定过程的稳定性。此外，Pt NPs和HRP显示出协同催化作用，提高了检测的灵敏度。此外，Zr- O的形成-P 键以可控方式消除了 PCN-224 和 iDNA 之间相互作用的不稳定性。免疫反应后，iDNA 刺激杂交链反应，导致上清液中荧光 DNA 显着减少，荧光信号发生变化。随后，PP-HRP-iDNA 探针实现了紫外光响应（450 nm），其中 3,3',5,5'-四甲基联苯胺用作比色信号读出的底物。凭借纳米材料调控的转导机制和抗原抗体相互作用，该双信号生物传感器具有高灵敏度，黄曲霉毒素 B 1的检测限为 0.65 pg/mL，肠炎沙门氏菌的检测限为4 CFU/mL, 表明生物传感平台在分析各种目标方面的检测潜力。
更新日期：2022-11-01
中文翻译：
“三合一”Zr-MOF 多功能载体介导的荧光和比色双信号读出生物传感平台可提高分析性能
为了满足对灵敏和稳定的检测应用的迫切需求，已经在开发用于精确目标检测和及时健康风险控制的双信号读出检测方面做出了重大努力。在这里，一种新的纳米材料 Pt@PCN-224-HRP-initiator DNA (PP-HRP-iDNA) 被用于构建双信号读出生物传感平台。Zr-MOF (PCN-224) 装载尽可能多的 Pt 纳米粒子 (NPs) 和辣根过氧化物酶 (HRP)，以增强肉眼可识别的比色信号的亮度，同时还充当守门人以保护酶活性并确保测定过程的稳定性。此外，Pt NPs和HRP显示出协同催化作用，提高了检测的灵敏度。此外，Zr- O的形成-P 键以可控方式消除了 PCN-224 和 iDNA 之间相互作用的不稳定性。免疫反应后，iDNA 刺激杂交链反应，导致上清液中荧光 DNA 显着减少，荧光信号发生变化。随后，PP-HRP-iDNA 探针实现了紫外光响应（450 nm），其中 3,3',5,5'-四甲基联苯胺用作比色信号读出的底物。凭借纳米材料调控的转导机制和抗原抗体相互作用，该双信号生物传感器具有高灵敏度，黄曲霉毒素 B 1的检测限为 0.65 pg/mL，肠炎沙门氏菌的检测限为4 CFU/mL, 表明生物传感平台在分析各种目标方面的检测潜力。
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