The hazelnut industry requires further development given the gap between production and demand. However, polyploid plantlet induction has not been reported in hazelnut varieties. As such, our aim in this study was to enrich the hazelnut germplasm resources. We used the shoot tips of in vitro propagated plantlets of Corylus heterophylla × Corylus avellana '84–226' as the material for inducing polyploidy. We supplemented the culture medium with 0, 100, 200, and 300 mg/L of colchicine, and we treated shoot tips for 20, 25, or 30 days. We found that the induction rate of tetraploids was the highest in treatments with 300 mg/L for 25 days; it was substantially higher by up to 20% than in the other treatments. In diploid and tetraploid tissue-cultured seedlings, tetraploid plantlets were much shorter, thicker, and stronger, as well as greener, than the diploid plantlets. The acquisition of polyploid hazelnuts provides an important prerequisite for hazelnut breeding.

鉴于产需之间的差距，榛子产业需要进一步发展。然而，在榛子品种中尚未报道多倍体植物诱导。因此，本研究旨在丰富榛子种质资源。我们使用了离体繁殖的太子木 × Corylus avellana幼苗的茎尖'84-226'作为诱导多倍体的材料。我们在培养基中添加了 0、100、200 和 300 mg/L 的秋水仙碱，我们将芽尖处理了 20、25 或 30 天。我们发现在300 mg/L 25天处理中四倍体的诱导率最高；它比其他处理方法高出 20%。在二倍体和四倍体组织培养的幼苗中，四倍体植株比二倍体植株更短、更厚、更强壮、更绿。多倍体榛子的获得为榛子育种提供了重要的先决条件。