Hydrogen peroxide (H2O2) is an important chemical substance produced in the metabolic process of organisms. Excess or less production could lead to serious effects on the body. Therefore, the development of advanced technology to accurately detect the content of H2O2 is of great significance. Herein, we developed a new ratiometric SERS nanoprobe based on shell-isolated nanoparticles (SHINs) for rapidly quantitative detection of H2O2. Because of the small Raman cross-section of H2O2, the ratiometric nanoprobe is an effective method for indirect detection of H2O2, which is designed based on the reaction of p-mercaptophenylboric acid (MPB) with H2O2 to form p-mercaptophenol (MP). Meanwhile, the nanoprobe was used to achieve quantitative detection of H2O2 and applied in quantitative detection of actual sample glucose, whose linear correlation coefficient could reach 0.9947 and 0.9812, respectively. This method expands the application of SERS technology, especially provides a reference for the detection of molecules with small Raman cross-section.

中文翻译：

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一种基于壳层分离纳米粒子快速定量测定过氧化氢的新型 SERS 方法

过氧化氢（H2O2）是生物体代谢过程中产生的一种重要化学物质。生产过多或过少都可能对身体造成严重影响。因此，开发先进的技术来准确检测H2O2的含量具有重要意义。在此，我们开发了一种基于壳分离纳米粒子 (SHIN) 的新型比率 SERS 纳米探针，用于快速定量检测 H2O2。由于 H2O2 的拉曼截面较小，因此基于对巯基苯硼酸 (MPB) 与 H2O2 反应生成对巯基苯酚 (MP) 而设计的比率纳米探针是间接检测 H2O2 的有效方法。同时，利用纳米探针实现H2O2的定量检测，并应用于实际样品葡萄糖的定量检测，其线性相关系数分别可达0.9947和0.9812。该方法拓展了SERS技术的应用，尤其为小拉曼截面分子的检测提供了参考。