CircNDST1 promotes papillary thyroid cancer progression via its interaction with CSNK2A1 to activate the PI3K–Akt pathway and epithelial–mesenchymal transition,Journal of Endocrinological Investigation

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CircNDST1 promotes papillary thyroid cancer progression via its interaction with CSNK2A1 to activate the PI3K–Akt pathway and epithelial–mesenchymal transition
Journal of Endocrinological Investigation ( IF 3.9 ) Pub Date : 2022-10-28 , DOI: 10.1007/s40618-022-01928-x
C Shu ₁ , S Wang ₁ , J Hu ₂ , M Xu ₁ , H Deng ₁ , Y Maimaiti ₃ , T Huang ₁

Affiliation

Background

Multiple studies have established a strong relationship between circRNA and cancer progression. Cervical lymph node metastasis is a key factor influencing the surgical approach and distant metastasis of papillary thyroid cancer (PTC). However, the role of circNDST1 in PTC has not been investigated. Our research focused on revealing the function and mechanism of action of circNDST1 in PTC.

Methods

High-throughput sequencing and qPCR were used to assess the expression of circRNA in PTC tissues with extensive cervical lymph node metastasis and circNDST1 in cell lines, respectively. The proliferative effects of circNDST1 in vitro and in vivo were analyzed using CCK8, clone formation assay, EdU, and nude mouse tumorigenesis assay. The transwell scratch assay was employed in the scrutiny of the effect of circNDST1 on the migration and invasion abilities of thyroid cancer cells, while circNDST1’s influence on the PI3K–Akt pathway and the Epithelial–Mesenchymal Transition (EMT) key protein expression was evaluated utilizing RNA sequencing and western blot. RNA pull-down and RIP were used to examine the binding of circNDST1 to CSNK2A1.

Results

CircNDST1 was highly expressed in PTC cell lines, but knocking it down inhibited the proliferation, migration, and invasive abilities of TPC1 and KTC1 cell lines. CircNDST1 bonded with CSNK2A1 and promoted the interaction between CSNK2A1 and Akt, leading to the activation of the PI3K–Akt pathway and EMT.

Conclusion

CircNDST1’s high expression boosted thyroid cancer progression through the activation of the PI3K–Akt pathway and EMT in a CSNK2A1-dependent manner

中文翻译：

CircNDST1 通过与 CSNK2A1 相互作用激活 PI3K-Akt 通路和上皮-间质转化促进甲状腺乳头状癌进展

背景

多项研究已经确定了 circRNA 与癌症进展之间的密切关系。颈部淋巴结转移是影响甲状腺乳头状癌(PTC)手术入路和远处转移的关键因素。然而，circNDST1 在 PTC 中的作用尚未得到研究。我们的研究重点是揭示 circNDST1 在 PTC 中的功能和作用机制。

方法

高通量测序和qPCR分别用于评估具有广泛颈部淋巴结转移的PTC组织中circRNA和细胞系中circNDST1的表达。使用CCK8、克隆形成试验、EdU和裸鼠肿瘤发生试验分析了circNDST1在体外和体内的增殖作用。采用transwell划痕实验考察circNDST1对甲状腺癌细胞迁移和侵袭能力的影响，同时利用RNA评估circNDST1对PI3K-Akt通路和上皮-间质转化（EMT）关键蛋白表达的影响测序和免疫印迹。RNA pull-down 和 RIP 用于检查 circNDST1 与 CSNK2A1 的结合。