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Real-Time Fluorescence Screening Platform for Meat Freshness
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-10-26 , DOI: 10.1021/acs.analchem.2c03326 Huan Ye 1 , Seyoung Koo 2 , Beitong Zhu 1 , Yingjun Ke 1 , Ruilong Sheng 3 , Ting Duan 1 , Lintao Zeng 1 , Jong Seung Kim 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-10-26 , DOI: 10.1021/acs.analchem.2c03326 Huan Ye 1 , Seyoung Koo 2 , Beitong Zhu 1 , Yingjun Ke 1 , Ruilong Sheng 3 , Ting Duan 1 , Lintao Zeng 1 , Jong Seung Kim 2
Affiliation
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Meat’s freshness is closely related to food safety and human health and has received increasing attention nowadays. To on-site visually screen meat freshness in a fast and non-destructive manner, we rationally constructed a series of fluorescent probes (JDCN, JDNS, and JDPY) with distinct electron-withdrawing substitution groups based on julolidine-fused coumarin. These probes underwent an aza-Michael addition followed by an elimination reaction with cadaverine to generate a colorimetric and ratiometric fluorescence response, and their sensing performance was rationally enhanced by improving the electron-withdrawing strength of substitution groups. Particularly, JDCN with a dicyanovinyl group as the reaction site exhibited outstanding sensing performance including rapid response (∼60 s), high selectivity, and low detection limit (14 nM). Furthermore, JDCN was fabricated into test kits to detect cadaverine vapor with a high-contrast fluorescence change from red to green. Based on two-color visualization of cadaverine vapor, on-site non-contact and non-destructive monitoring of meat freshness was successfully achieved. The good sensing performance rendered JDCN test kits a promising real-time fluorescence screening platform for rapid, non-destructive, and accurate evaluation of meat freshness.
中文翻译：
肉类新鲜度实时荧光筛查平台
肉类的新鲜度与食品安全和人体健康密切相关，越来越受到人们的关注。为了以快速、无损的方式现场目测肉类新鲜度，我们合理地构建了一系列基于朱洛利定融合香豆素的具有不同吸电子取代基团的荧光探针（ JDCN、JDNS和JDPY ）。这些探针经过氮杂-迈克尔加成，然后与尸胺发生消除反应，产生比色和比率荧光响应，并且通过提高取代基的吸电子强度合理地增强了它们的传感性能。特别是，JDCN以二氰基乙烯基为反应位点，表现出出色的传感性能，包括快速响应（~60 s）、高选择性和低检测限（14 nM）。此外，JDCN被制成测试套件，以检测具有从红色到绿色的高对比度荧光变化的尸胺蒸气。基于尸胺蒸气的双色可视化，成功实现了肉类新鲜度的现场非接触、无损监测。良好的传感性能使JDCN检测试剂盒成为快速、无损、准确评估肉类新鲜度的实时荧光筛选平台。
更新日期：2022-10-26
中文翻译：
肉类新鲜度实时荧光筛查平台
肉类的新鲜度与食品安全和人体健康密切相关，越来越受到人们的关注。为了以快速、无损的方式现场目测肉类新鲜度，我们合理地构建了一系列基于朱洛利定融合香豆素的具有不同吸电子取代基团的荧光探针（ JDCN、JDNS和JDPY ）。这些探针经过氮杂-迈克尔加成，然后与尸胺发生消除反应，产生比色和比率荧光响应，并且通过提高取代基的吸电子强度合理地增强了它们的传感性能。特别是，JDCN以二氰基乙烯基为反应位点，表现出出色的传感性能，包括快速响应（~60 s）、高选择性和低检测限（14 nM）。此外，JDCN被制成测试套件，以检测具有从红色到绿色的高对比度荧光变化的尸胺蒸气。基于尸胺蒸气的双色可视化，成功实现了肉类新鲜度的现场非接触、无损监测。良好的传感性能使JDCN检测试剂盒成为快速、无损、准确评估肉类新鲜度的实时荧光筛选平台。
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