榄仁多酚和近红外染料负载的聚（乳酸）纳米颗粒用于癌细胞的成像和光热治疗,ACS Applied Bio Materials

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榄仁多酚和近红外染料负载的聚（乳酸）纳米颗粒用于癌细胞的成像和光热治疗
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2022-10-26 , DOI: 10.1021/acsabm.2c00724
Monika Pebam , Rajalakshmi P.S. , Madhusree Gangopadhyay , Shashidhar Thatikonda , Aravind Kumar Rengan

光热/光动力疗法 (PTT/PDT) 是采用近红外 (NIR) 光响应光敏剂治疗癌症的多模式方法。在当前的研究中，IR-775 是一种疏水性光敏剂，与来自榄仁树的富含多酚 ( p ) 的乙酸乙酯提取物联合用于治疗癌症。IR-775 染料和多酚被封装在聚（乳酸）聚合物纳米系统（P p IR NPs）中以增加细胞生物利用度。P p IR NPs的流体动力学直径为 142.6 ± 2 nm，并表现出物理稳定性。该纳米系统在肺癌细胞系 (A549) 中表现出增强的细胞摄取。细胞毒性结果表明 P p与对照组相比，IR NPs 在 NIR 光处理后显示超过 82.46 ± 3% 的细胞死亡。PDT 和 PTT 都会产生活性氧 (ROS) 并引起体温过高，从而促进癌细胞死亡。定性和定量分析表明，近红外光照射下的 P p IR NPs 降低了 HSP70 和 PARP 的蛋白表达，并增加了 γ-H2AX，这些共同导致细胞死亡。近红外光照射后，HSP70 和 CDK2Na 的相对基因表达模式也下调。此外，已经在斑马鱼模型的 3D 细胞和卵内生物成像中研究了 P p IR NPs 摄取。总之，P pIR NPs 显示出良好的癌细胞细胞毒性，并为生物成像提供了潜在的纳米系统。

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Terminalia chebula Polyphenol and Near-Infrared Dye-Loaded Poly(lactic acid) Nanoparticles for Imaging and Photothermal Therapy of Cancer Cells

Photothermal/photodynamic therapies (PTT/PDT) are multimodal approaches employing near-infrared (NIR) light-responsive photosensitizers for cancer treatment. In the current study, IR-775, a hydrophobic photosensitizer, was used in combination with a polyphenols (p)-rich ethyl acetate extract from Terminalia chebula to treat cancer. IR-775 dye and polyphenols were encapsulated in a poly(lactic acid) polymeric nanosystem (PpIR NPs) to increase the cell bioavailability. The hydrodynamic diameter of PpIR NPs is 142.6 ± 2 nm and exhibited physical stability. The nanosystem showed enhanced cellular uptake in a lung cancer cell line (A549). Cell cytotoxicity results indicate that PpIR NPs showed more than 82.46 ± 3% cell death upon NIR light treatment compared to the control groups. Both PDT and PTT generate reactive oxygen species (ROS) and cause hyperthermia, thereby enhancing cancer cell death. Qualitative and quantitative analyses have depicted that PpIR NPs with NIR light irradiation have decreased protein expression of HSP70 and PARP, and increased γ-H2AX, which collectively lead to cell death. After NIR light irradiation, the relative gene expression patterns of HSP70 and CDK2Na were also downregulated. Further, PpIR NPs uptake has been studied in 3D cells and in ovo bioimaging in zebrafish models. In conclusion, the PpIR NPs show good cancer cell cytotoxicity and present a potential nanosystem for bioimaging.

更新日期：2022-10-26

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