交联胶原蛋白支架：抗感染潜力、亲水性和生物相容性,ACS Applied Polymer Materials

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交联胶原蛋白支架：抗感染潜力、亲水性和生物相容性
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2022-10-25 , DOI: 10.1021/acsapm.2c01627
Xuanqi Liu _{1,

2} , Xin Zheng _{1,

2} , Rongxin Feng _{1,

2} , Guofei Yu _{1,

2} , Na Yang _{1,

2} , Nianhua Dan _{1,

2} , Zhengjun Li ₁ , Weihua Dan _{1,

2} , Yining Chen _{1,

2}

Affiliation

猪脱细胞真皮基质（pADM）是一种具有优异生物学特性的基于胶原蛋白的医用支架材料。由于缺乏抗菌性，治疗过程中细菌引起的伤口感染极大地影响了患者的康复乃至生命安全。本文提出了一种基于自由基聚合反应的抗菌材料创新设计思路。采用两性单体甲基丙烯酸磺基甜菜碱（SBMA）与甲基丙烯酸酯化的pADM（MA-pADM）通过自由基聚合反应得到交联的两性离子接枝的pADM（C-MA-SBMA-pADM）。交联后的胶原蛋白支架具有足够的胶原蛋白稳定性、适当的力学性能、并保留了胶原蛋白的天然结构，同时引入了亲水的磺酸基和季铵基，通过水接触角检测，C-MA-SBMA-pADM表现出优异的亲水性。细胞实验结果表明该支架具有良好的细胞相容性。用大肠杆菌和金黄色葡萄球菌对材料的抗菌性能进行测试，结果表明交联的C-MA-SBMA-pADM具有良好的抗菌效果。综上所述，制备的交联胶原基支架具有良好的力学性能、细胞相容性和优异的抗菌性能，有望在组织工程支架材料领域得到更广泛的应用。

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Cross-Linked Collagen-Based Scaffold: Anti-infective Potential, Hydrophilic, and Biocompatible

Porcine decellularized dermal matrix (pADM) was a type of collagen-based medical scaffold material with excellent biological properties. Because of the lack of antibacterial properties, the wound infection caused by bacteria during the treatment process greatly affected the recovery and even the life safety of patients. In this paper, a creative design idea of antimicrobial material was proposed based on free radical polymerization reaction. The amphoteric monomer sulfobetaine methacrylate (SBMA) was employed to cross-link with methacrylated pADM (MA-pADM) by free radical polymerization to obtain cross-linked pADM grafted with amphoteric ions (C-MA-SBMA-pADM). The collagen scaffold after cross-linking had sufficient collagen stability, proper mechanical properties, and retained the natural structure of collagen while introducing hydrophilic sulfonic acid groups and quaternary ammonium groups, and C-MA-SBMA-pADM exhibited excellent hydrophilic properties by water contact angle detection. The results of cellular experiments showed that the scaffold had good cytocompatibility. Escherichia coli and Staphylococcus aureus were used to test the antibacterial properties of the material, and the results showed that the cross-linked C-MA-SBMA-pADM had good antibacterial effects. In conclusion, a cross-linked collagen-based scaffold was prepared with good mechanical properties, cytocompatibility, and also excellent antibacterial properties, which was expected to be more widely used in the field of tissue engineering scaffold materials.

更新日期：2022-10-25

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