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Phytoremediation of isoproturon-contaminated sites by transgenic soybean
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-10-24 , DOI: 10.1111/pbi.13951 Xiangkun Kong 1 , Na Lv 1 , Songmeng Liu 1 , Hui Xu 2 , Junwei Huang 3 , Xiangting Xie 4 , Qing Tao 4 , Baozhan Wang 1 , Rong Ji 5 , Qun Zhang 2 , Jiandong Jiang 1
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-10-24 , DOI: 10.1111/pbi.13951 Xiangkun Kong 1 , Na Lv 1 , Songmeng Liu 1 , Hui Xu 2 , Junwei Huang 3 , Xiangting Xie 4 , Qing Tao 4 , Baozhan Wang 1 , Rong Ji 5 , Qun Zhang 2 , Jiandong Jiang 1
Affiliation
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The widespread application of isoproturon (IPU) can cause serious pollution to the environment and threaten ecological functions. In this study, the IPU bacterial N-demethylase gene pdmAB was transferred and expressed in the chloroplast of soybean (Glycine max L. ‘Zhonghuang13’). The transgenic soybeans exhibited significant tolerance to IPU and demethylated IPU to a less phytotoxic metabolite 3-(4-isopropylphenyl)-1-methylurea (MDIPU) in vivo. The transgenic soybeans removed 98% and 84% IPU from water and soil within 5 and 14 days, respectively, while accumulating less IPU in plant tissues compared with the wild-type (WT). Under IPU stress, transgenic soybeans showed a higher symbiotic nitrogen fixation performance (with higher total nodule biomass and nitrogenase activity) and a more stable rhizosphere bacterial community than the WT. This study developed a transgenic (TS) soybean capable of efficiently removing IPU from its growing environment and recovering a high-symbiotic nitrogen fixation capacity under IPU stress, and provides new insights into the interactions between rhizosphere microorganisms and TS legumes under herbicide stress.
中文翻译：
转基因大豆对异丙隆污染场地的植物修复
异丙隆(IPU)的广泛应用会对环境造成严重污染并威胁生态功能。在本研究中，IPU细菌N-去甲基化酶基因pdmAB被转移并在大豆（ Glycine max L.‘中黄13’）叶绿体中表达。转基因大豆在体内表现出对IPU和去甲基化IPU对植物毒性较小的代谢物3-(4-异丙基苯基)-1-甲基脲(MDIPU)的显着耐受性。与野生型（WT）相比，转基因大豆在5天和14天内分别从水和土壤中去除了98%和84%的IPU，同时在植物组织中积累了更少的IPU。在IPU胁迫下，转基因大豆比野生型大豆表现出更高的共生固氮性能（具有更高的根瘤总生物量和固氮酶活性）和更稳定的根际细菌群落。这项研究开发了一种转基因（TS）大豆，能够有效地将IPU从其生长环境中去除，并在IPU胁迫下恢复高共生固氮能力，并为除草剂胁迫下根际微生物与TS豆科植物之间的相互作用提供了新的见解。
更新日期：2022-10-24
中文翻译：
转基因大豆对异丙隆污染场地的植物修复
异丙隆(IPU)的广泛应用会对环境造成严重污染并威胁生态功能。在本研究中，IPU细菌N-去甲基化酶基因pdmAB被转移并在大豆（ Glycine max L.‘中黄13’）叶绿体中表达。转基因大豆在体内表现出对IPU和去甲基化IPU对植物毒性较小的代谢物3-(4-异丙基苯基)-1-甲基脲(MDIPU)的显着耐受性。与野生型（WT）相比，转基因大豆在5天和14天内分别从水和土壤中去除了98%和84%的IPU，同时在植物组织中积累了更少的IPU。在IPU胁迫下，转基因大豆比野生型大豆表现出更高的共生固氮性能（具有更高的根瘤总生物量和固氮酶活性）和更稳定的根际细菌群落。这项研究开发了一种转基因（TS）大豆，能够有效地将IPU从其生长环境中去除，并在IPU胁迫下恢复高共生固氮能力，并为除草剂胁迫下根际微生物与TS豆科植物之间的相互作用提供了新的见解。
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