Oleandrin and adynerin are the main toxic components of oleander, an evergreen shrub or a small tree of the oleander family, which belongs to the class of cardiac glycosides exhibiting delayed action. The pharmacokinetic differences of oleandrin and adynerin in rats were studied by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) under two different administration modes: oral (5 mg/kg) and sublingual intravenous injection (1 mg/kg). The chromatographic column was UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm), and the column temperature was set at 40 °C. The mobile phase was acetonitrile–water (containing 0.1 % formic acid), with gradient elution, the flow rate was 0.4 mL/min, and the elution time was 4 min. Electrospray (ESI) positive ion mode detection with multiple reaction monitoring mode (MRM) was used for quantitative analysis: oleandrin m/z 577 → 145, adynerin m/z 534 → 113, and internal standard m/z 237 → 135. The established UPLC–MS/MS method was successfully applied to the pharmacokinetics in rats after administering oleandrin and adynerin. The bioavailability of oleandrin and adynerin was found to be low, 7.0 % and 93.1 %; respectively.

夹竹桃苷和夹竹桃苷是夹竹桃属常绿灌木或夹竹桃科小乔木的主要有毒成分，属于具有延迟作用的强心苷类。采用超高效液相色谱-串联质谱（UPLC-MS/MS）研究夹竹桃苷和腺苷脂在两种不同给药方式下的大鼠药代动力学差异：口服（5 mg/kg）和舌下静脉注射（1 mg/kg） ）。色谱柱为 UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm)，柱温设置为 40 °C。流动相为乙腈-水（含0.1%甲酸），梯度洗脱，流速0.4 mL/min，洗脱时间4 min。m / z 577 → 145，adynerin m / z 534 → 113，内标m / z 237 → 135。建立的 UPLC-MS/MS 方法成功应用于大鼠给药夹竹桃苷和 adynerin 后的药代动力学。夹竹桃苷和 adynerin 的生物利用度较低，分别为 7.0 % 和 93.1 %；分别。