Nanodiamond adsorption coatings were obtained on the surface of the collagen tissue crosslinked by glutaraldehyde for enhancing its mechanical properties, including stress at break, elongation at break, and elastic modulus (Young's modulus). The effect of nanodiamonds of positive and negative surface charge was compared. Negatively charged nanodiamonds strongly influenced the mechanical characteristics compared with positively charged particles on the background of its smaller surface concentration that was determined using tritium-labeled nanodiamonds. A chitosan layer was applied from the solution of supercritical CO₂ to prevent calcification of the tissue. In vivo experiments showed that the nanodiamond layer is stable and does not lead to additional calcification of the tissue. However, we observed higher metal concentrations on the tissue covered with negatively charged nanodiamonds. Note that the total calcification of bovine pericardium was less than for non-covered tissue. Our data revealed that layer-by-layer applied nanodiamond and chitosan films significantly enhance the mechanical properties of the bovine pericardium and allowed us to hope for the creation of a wear-resistant material based on it.

在戊二醛交联的胶原组织表面获得纳米金刚石吸附涂层，以增强其机械性能，包括断裂应力、断裂伸长率和弹性模量（杨氏模量）。比较了正负表面电荷的纳米金刚石的效果。与带正电的粒子相比，带负电的纳米金刚石强烈影响机械特性，因为其表面浓度较小，这是使用氚标记的纳米金刚石测定的。_{从超临界 CO 2}溶液中施加壳聚糖层以防止组织钙化。体内实验表明，纳米金刚石层是稳定的，不会导致组织的额外钙化。然而，我们观察到被带负电荷的纳米金刚石覆盖的组织上的金属浓度更高。请注意，牛心包的总钙化少于未覆盖的组织。我们的数据显示，逐层应用的纳米金刚石和壳聚糖薄膜显着增强了牛心包的机械性能，并让我们寄希望于创造一种基于它的耐磨材料。