The introduction of antibacterial and hemostatic hydrogels with good mechanical properties that display desirable impacts on wound healing process is still an unmet essential for clinical wound dressings. Herein, a multifunctional hydrogel PEG-CMC-THB-PRTM was fabricated via a one-pot method combining carboxymethyl chitosan (CMC), 2,3,4-trihydroxybenzaldehyde (THB), protamine (PRTM) and 4-arm polyethylene glycol aldehyde (PEG). The hydrogel was formed by the dynamic Schiff base reaction between amino groups of carboxymethyl chitosan and aldehyde groups of 4-arm polyethylene glycol aldehyde and exhibited excellent mechanical properties. The developed hydrogel also showed outstanding effects on anti-bacteria and hemostasis through the release of PRTM. Moreover, the hydrogel could promote extracellular matrix formation and wound closure in vivo, thereby accelerating the healing of skin wound. These results suggested that the multifunctional PEG-CMC-THB-PRTM hydrogel is a promising candidate for the clinical treatment of full-thickness wounds.

引入具有良好机械性能的抗菌和止血水凝胶，对伤口愈合过程显示出理想的影响，对于临床伤口敷料来说仍然是一个未满足的基本要求。在此，通过以下方法制备了多功能水凝胶 PEG-CMC-THB-PRTM结合羧甲基壳聚糖 (CMC)、2,3,4-三羟基苯甲醛 (THB)、鱼精蛋白 (PRTM) 和 4 臂聚乙二醇醛 (PEG) 的一锅法。该水凝胶由羧甲基壳聚糖的氨基与四臂聚乙二醇醛的醛基发生动态席夫碱反应形成，具有优异的机械性能。所开发的水凝胶还通过释放 PRTM 显示出出色的抗菌和止血效果。此外，水凝胶可以促进体内细胞外基质的形成和伤口闭合，从而加速皮肤伤口的愈合。这些结果表明多功能 PEG-CMC-THB-PRTM 水凝胶是临床治疗全层伤口的有前途的候选者。