The use of mushroom products as a dietary supplement is very common. One of these compounds includes the polysaccharides from the therapeutic fungus Fomes fomentarius, which has long been used to treat cancer. However, their effectiveness is poor due to their low solubility and stability. Solid lipid nanoparticles (SLNs) are nanocarriers that can enhance their effectiveness. In this study, Fomes fomentarius polysaccharides (EPS) and polysaccharides enriched with selenium (EPS-Se) were loaded into SLNs (SLN-EPS and SLN-EPS-Se). SLN synthesis was optimized using the response surface method (RSM) which showed that the drug/lipid and surfactant concentrations have significant effects (p < 0.05) on particle size and zeta potential. RSM results further showed that under optimal synthesis conditions (drug/lipid ratio: 0.062 (w/w), surfactant: 8.88 wt%), the particle size and zeta potential of these nanoparticles were 277.4 nm and −44.9 mV, respectively. Nanoparticle properties were further characterized with FITR, DSC, AFM, and FESEM. Lastly, the SLN-EPS-Se inhibited E. coli and S. aureus growth more than 50% and 78.5% after just 48 h (p < 0.05). SLN-EPS-Se inhibited the growth of gastric cancer cells AGS and MKN45 by 70% and 60%, respectively, by inducing necrosis, apoptosis, and disruption in cell cycles after 3 days. In summary, the results from this study suggest that the presently fabricated SLNs loaded with polysaccharides from Fomes fomentarius can be used as an improved drug supplement for cancer patients, especially those with weakened immune systems that suffer from bacterial infections.

使用蘑菇产品作为膳食补充剂非常普遍。其中一种化合物包括来自治疗性真菌Fomes fomentarius的多糖，该真菌长期以来一直用于治疗癌症。然而，由于它们的低溶解度和稳定性，它们的有效性很差。固体脂质纳米颗粒 (SLN) 是可以增强其有效性的纳米载体。在这项研究中，Fomes fomentarius将多糖（EPS）和富含硒的多糖（EPS-Se）加载到SLN（SLN-EPS和SLN-EPS-Se）中。使用响应面法 (RSM) 优化 SLN 合成，这表明药物/脂质和表面活性剂浓度对粒径和 zeta 电位具有显着影响 (p < 0.05)。RSM结果进一步表明，在最佳合成条件下（药物/脂质比：0.062（w/w），表面活性剂：8.88 wt%），这些纳米颗粒的粒径和zeta电位分别为277.4 nm和-44.9 mV。使用 FITR、DSC、AFM 和 FESEM 进一步表征了纳米颗粒的特性。最后，SLN-EPS-Se 抑制大肠杆菌和金黄色葡萄球菌仅 48 小时后增长超过 50% 和 78.5% (p < 0.05)。SLN-EPS-Se 通过在 3 天后诱导坏死、凋亡和细胞周期中断，分别抑制胃癌细胞 AGS 和 MKN45 的生长 70% 和 60%。总之，这项研究的结果表明，目前制造的含有大白斑多糖的 SLN可用作癌症患者的改进药物补充剂，尤其是那些患有细菌感染的免疫系统较弱的患者。