Acute response of biomarkers in plasma from capillary blood after a strenuous endurance exercise bout,European Journal of Applied Physiology

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Acute response of biomarkers in plasma from capillary blood after a strenuous endurance exercise bout
European Journal of Applied Physiology ( IF 2.8 ) Pub Date : 2022-10-13 , DOI: 10.1007/s00421-022-05068-1
Thomas Reichel ₁ , Steffen Held ₂ , Anthony Schwarz ₂ , Sebastian Hacker ₁ , Fabian Wesemann ₂ , Lars Donath ₂ , Karsten Krüger ₁

Affiliation

Purpose

The present study aims to investigate the acute response of potential exercise-sensitive biomarkers in capillary plasma to an acute incremental running test. In a second step, their concentration was compared to the changes in the venous serum.

Methods

Thirty-seven active young female and male adults completed a VO_2max ramp test on a treadmill. Before and after exercise, capillary blood from the earlobe and venous blood were taken and synchronized. Concentrations of Interleukin- (IL-) 1β, IL-1ra, IL-6, IL-8, IL-17A, Interferon (IFN)-y, CC-chemokine ligand (CCL)-2, Matrix metallopeptidase (MMP)-9, Secreted protein acidic and rich in cysteine (SPARC), Cluster of differentiation (CD)163, S100 Ca²⁺ -binding protein (S100) A8, S100A9, S100B, Brain-derived neurotrophic factor (BDNF), and Myeloperoxidase (MPO) were determined by magnetic bead-based multiplex assay.

Results

Capillary plasma concentrations of IL-1β, IL-6, IL-8, IL-17A, IFN-y, CCL-2, MMP-9, SPARC, CD163, S100A9, S100B, and BDNF increased after exercise (p < 0.05). Comparing the values from capillary plasma and venous serum, ICCs classified as good were found for IFN-y (post), while the ICCs for IL-1β, IL-8, IL-17A, CCL-2, MMP-9 (post), SPARC, and BDNF (post) were classified as moderate. For all other parameters, only weak ICCs were found.

Conclusion

As in the venous serum, there was an increase in most markers in the capillary plasma. However, acceptable to low associations can be found in the concentration levels of these proteins between the compartments. Thus, this source of blood sampling could find some biomarker applications in sports practice.

中文翻译：

剧烈耐力运动后毛细血管血血浆中生物标志物的急性反应

目的

本研究旨在研究毛细血管血浆中潜在的运动敏感生物标志物对急性增量跑步测试的急性反应。第二步，将它们的浓度与静脉血清的变化进行比较。

方法

37 名活跃的年轻女性和男性成年人在跑步机上完成了 VO _{2max斜坡测试。}运动前后，同步采集耳垂毛细血管血和静脉血。白介素- (IL-) 1β、IL-1ra、IL-6、IL-8、IL-17A、干扰素 (IFN)-y、CC-趋化因子配体 (CCL)-2、基质金属肽酶 (MMP)-9 的浓度, 富含半胱氨酸的酸性分泌蛋白 (SPARC), 分化簇 (CD)163, S100 Ca ²⁺结合蛋白 (S100) A8, S100A9, S100B, 脑源性神经营养因子 (BDNF), 髓过氧化物酶 (MPO)通过基于磁珠的多重测定法确定。

结果

IL-1β、IL-6、IL-8、IL-17A、IFN-y、CCL-2、MMP-9、SPARC、CD163、S100A9、S100B 和 BDNF 的毛细血管血浆浓度在运动后增加 ( p < 0.05) . 比较毛细血管血浆和静脉血清的值，发现 IFN-y 分类为良好的 ICC（后），而 IL-1β、IL-8、IL-17A、CCL-2、MMP-9 的 ICC（后）、SPARC 和 BDNF（后）被归类为中度。对于所有其他参数，仅发现较弱的 ICC。