Hydrophobic charge induction chromatography (HCIC) is becoming an attractive separation technology for antibody purification but suffers from low recovery. Herein we attempted to incorporate temperature responsiveness and strong ion exchange functions onto HCIC membrane adsorber to enhance antibody recovery. We proposed a multifunctional surface modification method combining surface-initiated reversible addition-fracture chain transfer polymerization (SI-RAFT) and the thiolactone chemistry. A regenerated cellulose (RC) membrane was modified by the SI-RAFT of N-isopropylacrylamide (NIPAAm) and thiolactone acrylamide (TIaAm), followed by the “amine-thiol-ene” cascade reaction of thiolactone with tryptamine, a HCIC ligand and methylpropylene trioxyethyl ammonium chloride (METAC), a strong ion exchanger. Temperature, pH and salt effects on IgG and BSA adsorption were investigated. After optimization, the RC-HCIC membrane adsorber had a static adsorption capacity of 41 mg/g for IgG, and an ideal IgG to BSA separation factor of 4.7, under the condition of pH = 7 and T = 30 °C. The membrane showed temperature-responsive IgG uptake from 10 °C to 35 °C. At pH = 5 and T = 10 °C, the recovery of IgG reached 93.5%, and the activity was well retained. These results indicate that the combination of temperature responsiveness of NIPAAm with HCIC is an effective strategy to improve the IgG recovery.

疏水电荷诱导色谱 (HCIC) 正在成为一种有吸引力的抗体纯化分离技术，但回收率较低。在这里，我们尝试将温度响应性和强离子交换功能结合到 HCIC 膜吸附器上，以提高抗体回收率。我们提出了一种结合表面引发的可逆加成-断裂链转移聚合（SI-RAFT）和硫代内酯化学的多功能表面改性方法。再生纤维素 (RC) 膜通过 N-异丙基丙烯酰胺 (NIPAAm) 和硫代内酯丙烯酰胺 (TIaAm) 的 SI-RAFT 改性，然后硫代内酯与色胺、HCIC 配体和甲基丙烯的“胺-硫醇-烯”级联反应三氧乙基氯化铵（METAC），一种强离子交换剂。温度，研究了 pH 值和盐对 IgG 和 BSA 吸附的影响。经过优化，RC-HCIC膜吸附器对IgG的静态吸附量为41 mg/g，在pH = 7和T = 30°C的条件下，IgG与BSA的理想分离因子为4.7。该膜在 10 °C 至 35 °C 范围内显示出对温度敏感的 IgG 摄取。在 pH = 5 和 T = 10 °C 时，IgG 的回收率达到 93.5%，活性保持良好。这些结果表明，NIPAAm 的温度响应性与 HCIC 的结合是提高 IgG 回收率的有效策略。该膜在 10 °C 至 35 °C 范围内显示出对温度敏感的 IgG 摄取。在 pH = 5 和 T = 10 °C 时，IgG 的回收率达到 93.5%，活性保持良好。这些结果表明，NIPAAm 的温度响应性与 HCIC 的结合是提高 IgG 回收率的有效策略。该膜在 10 °C 至 35 °C 范围内显示出对温度敏感的 IgG 摄取。在 pH = 5 和 T = 10 °C 时，IgG 的回收率达到 93.5%，活性保持良好。这些结果表明，NIPAAm 的温度响应性与 HCIC 的结合是提高 IgG 回收率的有效策略。