Tyrosinase from the banana peel (TBp) served as a low-cost immobilized biocatalyst for the production of 3, 4-di hydroxyphenyl-l-alanine (l-DOPA). l-DOPA, a dopamine precursor, useful in the management of Parkinson's disease, was produced in improvised reactors. l-DOPA production by the immobilized TBp was optimized via response surface methodology (RSM). l-DOPA productivities obtained at equimolar substrate concentrations and 500 mg of TBp for the packed bed and batch process (BP) were 34.0 ± 2.1 and 15.4 ± 0.9 mg/L/h respectively. The resulting l-DOPA productivity in the BP after the RSM model validation was 63 ± 3.0 mg/L/h. There was a good fit with the model obtained based on the closeness of the predicted and experimental values. After four successive batch processes, about 35% loss in l-DOPA productivity was observed. In conclusion, banana peel served as a relatively cheap and easily available source of tyrosinase for the production of l-DOPA in both batch and packed bed reactors.

香蕉皮中的酪氨酸酶 ( TBp ) 作为一种低成本的固定化生物催化剂用于生产 3, 4-二羟基苯基-L-丙氨酸 ( L- DOPA)。l -DOPA是一种多巴胺前体，可用于治疗帕金森病，是在简易反应器中生产的。通过响应面法 (RSM) 优化固定化TBp产生的l- DOPA。在等摩尔底物浓度和 500 mg TBp下，填充床和批处理 ( BP ) 获得的l- DOPA 产率分别为 34.0 ± 2.1 和 15.4 ± 0.9 mg/L/h。由此产生的l- DOPA 生产力在RSM 模型验证后的BP为 63 ± 3.0 mg/L/h。与基于预测值和实验值的接近度获得的模型有很好的拟合。在四个连续的分批过程之后，观察到L- DOPA 生产率损失约 35%。总之，香蕉皮是一种相对便宜且容易获得的酪氨酸酶来源，可用于在间歇式和填充床反应器中生产L- DOPA。