基于电阻的横向流动免疫传感器和支持 NFC 的智能手机，用于快速诊断临床样本中的钩端螺旋体病,Analytical Chemistry

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基于电阻的横向流动免疫传感器和支持 NFC 的智能手机，用于快速诊断临床样本中的钩端螺旋体病
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-10-11 , DOI: 10.1021/acs.analchem.2c02409
Sakda Jampasa ₁ , Chahya Kreangkaiwal ₂ , Kurt Kalcher ₃ , Wassa Waiwinya ₄ , Teerasit Techawiwattanaboon _{2,

5} , Nopporn Songumpai ₆ , Passorn Sueyanyongsiri ₇ , Kampol Pattanasombatsakul ₈ , Malee Techapornroong ₉ , Saovanee Benjamanukul ₁₀ , Orawon Chailapakul ₁₁ , Kanitha Patarakul _{2,

5} , Sudkate Chaiyo _{1,

11,

12}

Affiliation

Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok10330, Thailand.
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok10330, Thailand.
Institute of Chemistry, Analytical Chemistry, University of Graz, A-8010Graz, Austria.
Interdisciplinary Program, Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok10330, Thailand.
Chula Vaccine Research Center (Chula VRC), Center of Excellence in Vaccine Research and Development, Chulalongkorn University, Bangkok10330, Thailand.
Division of Infectious diseases, Department of Internal Medicine, Hatyai Hospital, Songkhla90110, Thailand.
Surin Hospital, Surin32000, Thailand.
Ranong Hospital, Ranong85000, Thailand.
Phrapokklao Hospital, Chantaburi22000, Thailand.
Department of Internal Medicine, Banphaeo General Hospital, Samut Sakhon74120, Thailand.
Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Chulalongkorn University, Bangkok10330, Thailand.
Center of Excellence for Food and Water Risk Analysis (FAWRA), Chulalongkorn University, Bangkok10330, Thailand.

钩端螺旋体病是由致病性钩端螺旋体引起的最危及生命的热带疾病之一. 迄今为止，仍然需要一种能够快速、灵敏地检测钩端螺旋体的诊断设备，以进行适当的治疗以降低死亡率。在此，我们创建了一种基于电阻的横向流动免疫传感器诊断设备 (R-LFI)，它将近场通信 (NFC) 与便携式智能手机集成在一起，用于临床样本中的钩端螺旋体检测。一种针对病原体的特异性单克隆抗体被涂在硝酸纤维素膜 (NCM) 上，测试线位于该膜上。使用导电双面胶带安装了两个具有三明治状配置的电极，并与基于 NFC 智能手机的检测系统连接。半夹心免疫复合物形成诱导高质子传导，导致电阻反应显着降低。问号钩端螺旋体和临床样本。R-LFI 设备对磷酸盐缓冲液中的 rLipL32 蛋白和L. interrogans的健康人血清样品的浓度范围为 1 至 1000 ng mL ^–1（检测限 (LOD)：0.29 ng mL ^–1）表现出线性响应和 10 ⁴至 10 ⁶细胞 mL ^–1（LOD：4.89 × 10 ³细胞 mL ^–1）。我们的 R-LFI 传感器成功检测到问号乳杆菌-通过聚合酶链反应 (PCR) 确认的阳性临床样本。该平台具有高特异性、选择性、简单性、极小的样品量，并且不需要标记元素。这些理想的特性使其特别适用于医疗设施和资源有限的国家。

"点击查看英文标题和摘要"

Resistance-Based Lateral Flow Immunosensor with a NFC-Enabled Smartphone for Rapid Diagnosis of Leptospirosis in Clinical Samples

Leptospirosis is one of the most life-threatening tropical diseases caused by pathogenic Leptospira. To date, a diagnostic device that offers rapid and sensitive detection of leptospires has been still in demand for proper treatment to reduce the mortality rate. Herein, we create a resistance-based lateral flow immunosensor diagnosis device (R-LFI) that integrates near-field communication (NFC) with a portable smartphone for leptospiral detection in clinical samples. A specific monoclonal antibody against the pathogen was coated on a nitrocellulose membrane (NCM) where the test line was collocated. Two electrodes with a sandwich-like configuration were installed employing a conductive double-sided adhesive tape and connected with a NFC smartphone-based detection system. A half-sandwich immunocomplex formation induced high proton conduction, resulting in a considerable decrement in resistive response. The performance of the R-LFI sensor was evaluated using recombinant LipL32 (rLipL32), Leptospira interrogans, and clinical samples. The R-LFI device exhibited linear responses toward rLipL32 protein in phosphate buffer and L. interrogans-spiked healthy human serum samples within the concentration ranging from 1 to 1000 ng mL^–1 (limit of detection (LOD): 0.29 ng mL^–1) and from 10⁴ to 10⁶ cell mL^–1 (LOD: 4.89 × 10³ cell mL^–1), respectively. Our R-LFI sensor successfully detected L. interrogans-positive clinical samples as confirmed by polymerase chain reaction (PCR). This platform offers high specificity, selectivity, simplicity, miniscule sample volume, and no labeling element requirement. These desirable features make it particularly suitable for countries where medical facilities and resources are limited.

更新日期：2022-10-11

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