SPINDLY 与 EIN2 相互作用促进乙烯信号介导的番茄果实成熟,Plant Biotechnology Journal

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SPINDLY 与 EIN2 相互作用促进乙烯信号介导的番茄果实成熟
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-10-06 , DOI: 10.1111/pbi.13939
Jin Xu ₁ , Sidi Liu ₁ , Licong Cai ₁ , Lingyu Wang ₁ , Yufei Dong ₁ , Zhenyu Qi ₂ , Jingquan Yu _{1,

3} , Yanhong Zhou _{1,

3,

4}

Affiliation

蛋白质的翻译后修饰使细胞能够通过控制蛋白质功能对动态刺激做出迅速反应。在高等植物中，SPINDLY (SPY) 和 SECRET AGENT (SEC) 是两种主要的O-糖基化酶，它们具有独特和重叠的作用；然而，它们的O-糖基化对果实成熟的影响及其潜在机制在很大程度上仍然未知。在这里，我们报告 SlSPY 影响番茄果实成熟。使用slspy突变体和两个SlSPY-OE 品系，我们提供了 SlSPY 在果实成熟中的积极作用的生物学证据。我们证明 SlSPY 通过改变番茄中的乙烯反应来调节果实成熟。为了进一步研究其潜在机制，我们将乙烯信号传导乙烯不敏感 2 (EIN2) 的中央调节器鉴定为 SlSPY 相互作用蛋白。SlSPY 促进 SlEIN2 的稳定性和核积累。质谱分析进一步确定SlEIN2具有两个潜在的O-聚糖修饰位点Ser771和Thr821 。进一步的研究表明，SlEIN2 对 SlSPY 调控番茄果实成熟至关重要。总的来说，我们的研究结果揭示了 SlSPY 在果实中的一种新的调节功能，并为 SlSPY-SlEIN2 模块在番茄果实成熟中的作用提供了新的见解。

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SPINDLY interacts with EIN2 to facilitate ethylene signalling-mediated fruit ripening in tomato

The post-translational modification of proteins enables cells to respond promptly to dynamic stimuli by controlling protein functions. In higher plants, SPINDLY (SPY) and SECRET AGENT (SEC) are two prominent O-glycosylation enzymes that have both unique and overlapping roles; however, the effects of their O-glycosylation on fruit ripening and the underlying mechanisms remain largely unknown. Here we report that SlSPY affects tomato fruit ripening. Using slspy mutants and two SlSPY-OE lines, we provide biological evidence for the positive role of SlSPY in fruit ripening. We demonstrate that SlSPY regulates fruit ripening by changing the ethylene response in tomato. To further investigate the underlying mechanism, we identify a central regulator of ethylene signalling ETHYLENE INSENSITIVE 2 (EIN2) as a SlSPY interacting protein. SlSPY promotes the stability and nuclear accumulation of SlEIN2. Mass spectrometry analysis further identified that SlEIN2 has two potential sites Ser771 and Thr821 of O-glycans modifications. Further study shows that SlEIN2 is essential for SlSPY in regulating fruit ripening in tomatoes. Collectively, our findings reveal a novel regulatory function of SlSPY in fruit and provide novel insights into the role of the SlSPY-SlEIN2 module in tomato fruit ripening.

更新日期：2022-10-06

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