Heterostructure Cu₂O@CuO nanosheets are spontaneously formed during the kinetic-spraying of nanostructured CuO thin film on the fluorine-doped tin oxide (FTO) substrate at room temperature under low vacuum conditions by the NPDS technique. The Cu₂O@CuO nanosheets fabricated as a heterogeneous electrocatalyst are utilized for free enzyme detection of glucose and H₂O₂ in 0.1 M NaOH, whereby the synergy between the multivalent copper species (I and II) results in an overall improvement of electrocatalytic performance. The in-situ formation of interfacial Cu₂O@CuO heterostructure is verified using a high-resolution transmission microscope and X-ray photon electron spectroscopy. The fabricated electrocatalysts exhibit high sensitivity toward glucose oxidation of 818.5 μA·mM⁻¹·cm⁻² with a wide linear detection range extending from 0.02 to 5 mM and a limit of detection (LOD) of 5.8 μM. Also, the heterostructure Cu₂O@CuO nanosheets show a very wide linear detection range of H₂O₂ oxidation from 0.02 to 11 mM with a good detection sensitivity of 168 μA·mM⁻¹·cm⁻² and a LOD of 8.75 μM. The long-term stability for around 30 min and high oxidation selectivity with other interfering species are verified. Furthermore, Cu₂O@CuO nanosheets exhibit higher catalytic rate constant and diffusion coefficient toward glucose species compared with H₂O₂.

异质结构 Cu ₂ O@CuO 纳米片是在室温下在低真空条件下通过 NPDS 技术在氟掺杂氧化锡 (FTO) 基板上动态喷涂纳米结构 CuO 薄膜的过程中自发形成的。作为非均相电催化剂制造的 Cu ₂ O@CuO 纳米片可用于在 0.1 M NaOH 中检测葡萄糖和 H ₂ O ₂的游离酶，由此多价铜物种（I 和 II）之间的协同作用导致电催化性能的整体改善表现。_{界面Cu 2}的原位形成使用高分辨率透射显微镜和 X 射线光子电子能谱验证 O@CuO 异质结构。制备的电催化剂对葡萄糖氧化表现出818.5 μA·mM ^-1 ·cm ^-2的高灵敏度，具有从0.02 到5 mM 的宽线性检测范围和5.8 μM 的检测限（LOD）。此外，异质结构Cu _{2 O@CuO纳米片显示出从0.02到11 mM的非常宽的H 2} O ₂氧化线性检测范围，具有168 μA·mM ^-1 ·cm ^-2的良好检测灵敏度和8.75 μM的LOD。 . 验证了约 30 分钟的长期稳定性和与其他干扰物质的高氧化选择性。此外，铜_{与H 2} O _{2相比， 2} O@CuO 纳米片对葡萄糖种类表现出更高的催化速率常数和扩散系数。