Selective and Efficient Capture and Release of vic-Diol-Containing Pacific and Caribbean Ciguatoxins from Fish Extracts with a Boronate Affinity Polymer,Journal of Agricultural and Food Chemistry

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Selective and Efficient Capture and Release of vic-Diol-Containing Pacific and Caribbean Ciguatoxins from Fish Extracts with a Boronate Affinity Polymer
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-10-03 , DOI: 10.1021/acs.jafc.2c03789
Elizabeth M Mudge ₁ , Alison Robertson _{2,

3} , Pearse McCarron ₁ , Christopher O Miles ₁

Affiliation

Ciguatera poisoning can occur following the consumption of fish contaminated with trace levels of ciguatoxins (CTXs). These trace levels represent an analytical challenge for confirmation by LC–MS due to matrix interferences and the high instrument sensitivity required. Sample preparation procedures are laborious and require extensive cleanup procedures to address these issues. The application of a selective isolation technique employing boronate affinity polymers was therefore investigated for the capture of vic-diol-containing Caribbean and Pacific CTXs from fish extracts. A dispersive SPE procedure was developed where nearly complete binding of CTXs in fish extracts occurred with boric acid gel in less than 1 h. Release of the bound CTXs resulted in >95% recovery of C-CTX1/2, C-CTX3/4, CTX1B, 54-deoxyCTX1B, and 52-epi-54-deoxyCTX1B from the extracts. This selective extraction tool has the potential to greatly simplify both analytical sample preparation and preparative extraction and isolation of CTXs for structure elucidation and production of standards.

中文翻译：

使用硼酸盐亲和聚合物从鱼类提取物中选择性高效捕获和释放含有 vic-Diol 的太平洋和加勒比雪卡毒素

食用被微量雪卡毒素 (CTX) 污染的鱼后，可能会发生雪卡中毒。由于基质干扰和所需的高仪器灵敏度，这些痕量水平代表了 LC-MS 确认的分析挑战。样品制备程序很费力，需要大量的清理程序来解决这些问题。因此，研究了采用硼酸盐亲和聚合物的选择性分离技术来捕获vic-来自鱼类提取物的含有二醇的加勒比和太平洋 CTX。开发了分散 SPE 程序，其中在不到 1 小时的时间内，用硼酸凝胶几乎完全结合了鱼提取物中的 CTX。结合的 CTX 的释放导致提取物中 C-CTX1/2、C-CTX3/4、CTX1B、54-deoxyCTX1B 和 52 - epi -54-deoxyCTX1B 的回收率 >95% 。这种选择性提取工具有可能极大地简化分析样品制备以及 CTX 的制备提取和分离，以进行结构解析和标准品生产。

更新日期：2022-10-03

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