Dyes and Pigments ( IF 4.1 ) Pub Date : 2022-10-01 , DOI: 10.1016/j.dyepig.2022.110796
Xu-Chen Feng , Gang Zhang , Ru Sun , Yu-Jie Xu , Jian-Feng Ge
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As an important organelle, the main function of the Golgi apparatus is to further modify and deliver proteins synthesized by the endoplasmic reticulum (ER). Dyshomeostasis of the Golgi appartus can cause many serious diseases. Therefore, monitoring the normal functioning of the Golgi appartus is particularly important. However, only a few commercially Golgi markers are available till now. In this work, three 1,8-naphthalimide related Golgi markers (4a-c) with the combination of amide and long carbon chain were designed and synthesized, their maximum emission wavelengths could reach 500–575 nm with high fluorescence quantum yields. Moreover, they possessed low cytotoxicity (cell viabilities >82%). Cell experiments exhibited that dyes 4a-c could rapidly and accurately mark the Golgi apparatus. To discuss the origin of their targeting ability, compounds 5–7 with independent amide or long carbon chain were additionally prepared. With the separation of amide and long carbon chain in compound 5–7, the targeting ability was lost. The experiments showed that the targeting ability of 4a-c resulted from the combination of amide and long carbon chain structure. Also, the results may provide a new idea for designing organelle markers.
中文翻译:
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几种基于具有酰胺和长碳链的 1,8-萘酰亚胺衍生物的高尔基体靶向荧光标记物
作为重要的细胞器,高尔基体的主要功能是进一步修饰和传递内质网(ER)合成的蛋白质。高尔基体的平衡失调会导致许多严重的疾病。因此,监测高尔基体的正常功能尤为重要。然而,到目前为止,只有少数商业高尔基标记可用。本工作设计并合成了三个酰胺和长碳链结合的1,8-萘酰亚胺相关高尔基体标记物(4a - c ) ,它们的最大发射波长可达500-575 nm,具有较高的荧光量子产率。此外,它们具有低细胞毒性(细胞活力> 82%)。细胞实验表明染料4a-c可以快速准确地标记高尔基体。为了讨论它们靶向能力的来源,另外制备了具有独立酰胺或长碳链的化合物5-7 。随着化合物5-7中酰胺和长碳链的分离,靶向能力丧失。实验表明,4a - c的靶向能力来源于酰胺和长碳链结构的结合。此外,该结果可能为设计细胞器标记提供新思路。