黄芩苷通过钙-CHOP 途径调节 LPS 激活的巨噬细胞的炎症反应,Cells

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黄芩苷通过钙-CHOP 途径调节 LPS 激活的巨噬细胞的炎症反应
Cells ( IF 5.1 ) Pub Date : 2022-09-30 , DOI: 10.3390/cells11193076
Hyo-Jin An ₁ , Ji-Young Lee ₂ , Wansu Park ₂

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对可以减轻内毒素（脂多糖）引起的巨噬细胞炎症反应的天然产物的研究仍在继续。本研究调查了黄芩苷与脂多糖 (LPS) 引起的巨噬细胞活化相关的抗炎活性。黄芩苷是一种黄酮苷，存在于黄芩属黄芩和黄芩属植物中. 使用 RAW 264.7 小鼠巨噬细胞进行多重细胞因子测定 (MCA)、Griess 试剂测定、fluo-4 钙测定、二氢罗丹明 123 (DHR123) 测定、定量 RT-PCR 和流式细胞术。MCA 显示黄芩苷显着降低白细胞介素 (IL)-6、粒细胞集落刺激因子 (G-CSF)、血管内皮生长因子 (VEGF)、巨噬细胞炎症蛋白 (MIP)-1α、MIP-1β、MIP 的产生LPS 刺激的 RAW 264.7 巨噬细胞中的 -2 和 RANTES，浓度分别为 10、25 和 50 μM。DHR123 测定显示黄芩苷显着抑制 LPS 刺激的 RAW 264.7 巨噬细胞中活性氧的产生。流式细胞术显示黄芩苷显着降低 LPS 刺激的 RAW 264.7 巨噬细胞中磷酸化 p38 MAPK 和 Fas 的水平。Chop、Fas、Nos2、Ptgs2、Stat1、c-Jun、c-Fos和At1a。黄芩苷对 IL-6、TNF-α、G-CSF、VEGF、干扰素γ诱导蛋白 10 (IP-10)、白血病抑制因子 (LIF)、脂多糖诱导的 CXC 趋化因子 (LIX)、MIP的 IC ₅₀值-1α、MIP-1β、MIP-2、RANTES、一氧化氮、细胞内钙和过氧化氢分别为 591.3、450、1719、27.68、369.4、256.6、230.7、856.9、1326、1524、378.1、26.76、345.1 和分别为 32.95 μM。黄芩苷通过钙-CHOP 途径调节被 LPS 激活的巨噬细胞的炎症反应。

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Baicalin Modulates Inflammatory Response of Macrophages Activated by LPS via Calcium-CHOP Pathway

Studies on natural products that can alleviate the inflammatory response of macrophages caused by endotoxin (lipopolysaccharide) continue. This study investigated the anti-inflammatory activity of baicalin related to macrophage activation caused by lipopolysaccharide (LPS). Baicalin is a flavone glycoside found in plants such as Scutellaria baicalensis and Scutellaria lateriflora belonging to the genus Scutellaria. The multiplex cytokine assay (MCA), Griess reagent assay, fluo-4 calcium assay, dihydrorhodamine 123 (DHR123) assay, quantitative RT-PCR, and flow cytometry were performed using RAW 264.7 mouse macrophages. The MCA revealed that baicalin significantly decreased the production of interleukin (IL)-6, granulocyte colony-stimulating factor (G-CSF), vascular endothelial growth factor (VEGF), macrophage inflammatory protein (MIP)-1α, MIP-1β, MIP-2, and RANTES in LPS-stimulated RAW 264.7 macrophages at concentrations of 10, 25, and 50 μM. The DHR123 assay showed that baicalin significantly inhibited reactive oxygen species generation in LPS-stimulated RAW 264.7 macrophages. Flow cytometry revealed that baicalin significantly reduced the levels of phosphorylated p38 MAPK and Fas in LPS-stimulated RAW 264.7 macrophages. Baicalin also inhibited the mRNA expression levels of inflammatory genes such as Chop, Fas, Nos2, Ptgs2, Stat1, c-Jun, c-Fos, and At1a. The IC₅₀ values of baicalin for IL-6, TNF-α, G-CSF, VEGF, interferon gamma-induced protein 10 (IP-10), leukemia inhibitory factor (LIF), lipopolysaccharide-induced CXC chemokine (LIX), MIP-1α, MIP-1β, MIP-2, RANTES, nitric oxide, intracellular calcium, and hydrogen peroxide were 591.3, 450, 1719, 27.68, 369.4, 256.6, 230.7, 856.9, 1326, 1524, 378.1, 26.76, 345.1, and 32.95 μM, respectively. Baicalin modulated the inflammatory response of macrophages activated by LPS via the calcium-CHOP pathway.

更新日期：2022-09-30

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