Microchimica Acta ( IF 5.3 ) Pub Date : 2022-09-30 , DOI: 10.1007/s00604-022-05488-y Wangbin Zhang 1 , Qianyu Zhao 2 , Xiaoqin Zhou 1 , Jie Chen 1 , Yong Liu 1 , Liping Tang 1 , Jianxin Min 1 , Dongmei Yan 1 , Chen Xu 2 , Bin Li 1 , Xin Yang 2
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Magnetic molecular imprinted polymers (MIPs) based on 4-vinylbenzyltrimethylammonium chloride (VBTAC) and 4-vinylbenzoic acid (VBA) deep eutectic solvent as dual functional monomers was successfully synthesized for the specific recognition of laminarin. The MIPs were characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, thermal gravimetric analysis, and vibrating sample magnetometer analysis. The results showed that the MIPs were spheres of a uniform size, with the surface rich in cavities and excellent superparamagnetism properties. The adsorption experiments showed that MIPs conform to pseudo-second-order kinetics and Langmuir isotherm adsorption. The maximum adsorption capacity under optimal conditions was 322.58 μg·mg−1 and the imprinting factor was 2.13. Under the optimized conditions, the limit of detection (LOD) of the developed material was 6.6 µM. Linearity of the material was obtained within the range 20–800 µM with a coefficient of determination (r2) being better 0.999. Relative standard deviations (RSDs) were less than 3.96%, and satisfactory recoveries were between 94.55 and 97.39%. The actual sample analysis manifested that MIPs could effectively separate laminarin from Laminarin japonica Aiesch.
Graphical abstract
中文翻译：
一种用于从海藻中提取昆布多糖的深共晶溶剂磁性分子印迹聚合物
成功合成了基于4-乙烯基苄基三甲基氯化铵（VBTAC）和4-乙烯基苯甲酸（VBA）深共熔溶剂作为双功能单体的磁性分子印迹聚合物（MIPs），用于特异性识别昆布多糖。通过透射电子显微镜、扫描电子显微镜、傅里叶变换红外光谱、热重分析和振动样品磁力计分析对 MIP 进行了表征。结果表明，MIPs为大小均匀的球体，表面富含空腔，具有优异的超顺磁性。吸附实验表明MIPs符合准二级动力学和Langmuir等温吸附。最佳条件下的最大吸附量为322.58 μg·mg -1印迹因子为2.13。在优化条件下，开发材料的检测限 (LOD) 为 6.6 µM。在 20–800 µM 范围内获得了材料的线性，确定系数 ( r 2 ) 更好为 0.999。相对标准偏差 (RSD) 小于 3.96%，令人满意的回收率在 94.55 和 97.39% 之间。实际样品分析表明，MIPs可以有效地将海带多糖与海带多糖分离。
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