超高效液相色谱-串联质谱法测定化妆品中的L-麦角硫因,International Journal of Analytical Chemistry

当前位置： X-MOL 学术 › Int. J. Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

超高效液相色谱-串联质谱法测定化妆品中的L-麦角硫因
International Journal of Analytical Chemistry ( IF 1.5 ) Pub Date : 2022-9-27 , DOI: 10.1155/2022/4372295
Li Liu ₁ , Lei Sun ₂ , Sufang Fan ₂ , Junmei Ma ₂ , Yi Wang ₃ , Qiang Li ₂ , Zhengyang Song ₃ , Yong Sun ₁ , Yan Zhang ₂

Affiliation

建立了一种基于超高效液相色谱-串联质谱（UPLC-MS/MS）的化妆品中L-麦角硫因的鉴定和测定新方法。优化了化妆品样品的预处理方法、色谱柱、色谱条件和质谱条件。选择甲醇作为萃取溶剂，选择含 0.1% FA 的 85% 乙腈作为流动相，选择 Waters CORTECS UPLC 亲水相互作用液相色谱 (HILIC) 柱进行分离。样品用甲醇萃取，过滤，HILIC 分离，三重四极杆质谱检测。使用外标法在基质校准曲线下进行定量。结果显示在5~200 ng/mL范围内具有良好的线性关系，化妆品样品中相关系数大于0.999。检测限在 25-50 范围内 μ g/kg，定量限在 50-100 μ g/kg 范围内。加标方法的回收率范围为 85.3% 至 96.2%，相对标准偏差 (RSD) 范围为 0.84%–2.08% ( n = 6)。该方法简便、快速、准确，用于化妆品中L-麦角硫因的测定，具有很大的实用价值。

"点击查看英文标题和摘要"

Determination of L-Ergothioneine in Cosmetics Based on Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry

A new method was developed for the identification and determination of L-ergothioneine in cosmetics based on ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The pretreatment method, chromatographic column, chromatographic conditions, and mass spectrometric conditions of cosmetic samples were optimized. Methanol was chosen as the extraction solvent, 85% acetonitrile with 0.1% FA was selected as the mobile phase, and the Waters CORTECS UPLC hydrophilic interaction liquid chromatography (HILIC) column was chosen for the separation. The sample was extracted with methanol and filtered, then separated by HILIC and detected by triple-quadrupole mass spectrometry. The quantitation was done under the matrix calibration curve using the external standard method. The results showed good linear relationships in the range of 5–200 ng/mL, and the correlation coefficient was greater than 0.999 in cosmetic samples. The limit of detection was in the range of 25–50 μg/kg and the limit of quantitation was in the range of 50–100 μg/kg. The recoveries of the method spiked ranged from 85.3% to 96.2% and the relative standard deviation (RSD) was in the range of 0.84%–2.08% (n = 6). The method is simple, quick, and accurate for the determination of L-ergothioneine in cosmetics, and has great practical value.

更新日期：2022-09-28

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南