In this work, personal glucose meter (PGM) as a portable electrochemical device was utilized for sensitive detection of non-glucose targets: N-gene and PCB77, respectively. DNA hydrogel, which can respond to CRISPR/Cas system, was prepared for label-free encapsulating invertase. In the presence of targets, the repeated sequence for the activation of Cas12a was obtained due to the performance of RCA. Unlike “one-to-one” recognition, activated Cas12a can efficiently cleave multiple single-stranded linker DNAs on DNA hydrogels, thus releasing many invertase that can be used for PGM detection. With the amplification of RCA and CRISPR/Cas system, high detection sensitivity can be obtained even using portable PGM. The detection limits for N-gene and PCB77 were 2.6 fM and 3.2 × 10⁻⁵ μg/L, respectively, with high specificity and good practical application performance. The developed biosensor can be used for online monitoring with the merit of low cost, easy operation and can be used for various targets analysis.

在这项工作中，个人血糖仪（PGM）作为一种便携式电化学装置被用于灵敏检测非葡萄糖目标：N基因和PCB77，分别。可响应CRISPR/Cas系统的DNA水凝胶被制备用于无标记包封转化酶。在存在靶标的情况下，由于 RCA 的性能，获得了激活 Cas12a 的重复序列。与“一对一”识别不同，激活的 Cas12a 可以有效地切割 DNA 水凝胶上的多个单链接头 DNA，从而释放出许多可用于 PGM 检测的转化酶。通过 RCA 和 CRISPR/Cas 系统的放大，即使使用便携式 PGM 也可以获得高检测灵敏度。N基因和PCB77的检测限为2.6 fM和3.2 × 10 ^-5 μg/L 分别具有高特异性和良好的实际应用性能。开发的生物传感器可用于在线监测，具有成本低、操作简单、可用于各种目标分析的优点。