基于介孔钙掺杂MgAl2O3-G-SiO2生物传感器的大肠杆菌O157:H7电化学发光检测,New Journal of Chemistry

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基于介孔钙掺杂MgAl2O3-G-SiO2生物传感器的大肠杆菌O157:H7电化学发光检测
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2022-09-27 , DOI: 10.1039/d2nj03870f
Ze-da Meng ₁ , Chong-Hun Jung ₂ , Won-Chun Oh _{3,

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Affiliation

本文介绍了基于生物相容性介孔 Ca 掺杂 MgAl ₂ O ₃ -G-SiO ₂ (CMAGS) 纳米材料的基于石墨烯的介孔电化学生物传感器的开发，用于定量检测大肠杆菌( E. coli) O157:H7。使用各种技术对纳米复合材料进行形态学表征，例如 XRD、SEM、BET、HRTEM、拉曼、DRS 和 XPS。此外，用于传感测试的活性材料使用循环伏安法 (CV) 进行电化学表征。通过自组装路线成功建立了大肠杆菌O157:H7的均匀膜。目标细菌被电极直接感应，从而允许大肠杆菌O157:H7 被选择性识别；使用循环伏安传感系统检测大肠杆菌O157:H7。在最佳条件下，大肠杆菌O157:H7 浓度之间的线性关系从 10 ¹ CFU 到 10 ¹⁰ CFU 获得，检测限 (LOD)为 10 ^{1 CFU。}该生物传感器还成功地用于水样的检测。一种基于介孔 Ca 掺杂 MgAl ₂ O ₃ -G-SiO ₂ (CMAGS) 的大肠杆菌传感方法O157:H7 首次被报道。该传感系统对目标分析物显示出高选择性，有望带来新的机遇，例如加快疾病诊断、治疗和病原体预防。

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Electrochemiluminescence detection of Escherichia coli O157:H7 based on mesoporous Ca-doped MgAl2O3–G–SiO2 biosensor

This paper describes the development of a graphene-based mesoporous electrochemical biosensor for the quantitative detection of Escherichia coli (E. coli) O157:H7 based on biocompatible mesoporous Ca-doped MgAl₂O₃–G–SiO₂ (CMAGS) nanomaterials. The nanocomposites were morphologically characterized using various techniques, such as XRD, SEM, BET, HRTEM, Raman, DRS, and XPS. In addition, the active materials for the sensing test were electrochemically characterized using cyclic voltammetry (CV). The uniform film for E. coli O157:H7 was successfully established through a self-assembly route. The target bacteria were directly sensed by the electrode, thus allowing E. coli O157:H7 to be selectively recognized; E. coli O157:H7 was detected using the cyclic voltammetry sensing system. Under optimal conditions, the linear relationships between E. coli O157:H7 concentrations were obtained from 10¹ CFU to 10¹⁰ CFU with a 10¹ CFU limit of detection (LOD). The biosensor was also successfully used in the detection of water samples. A mesoporous Ca-doped MgAl₂O₃–G–SiO₂ (CMAGS)-based sensing method for E. coli O157:H7 was reported for the first time. The sensing system showed high selectivity to the target analyte and is expected to lead to new opportunities, such as speeding up disease diagnosis, treatment, and prevention with pathogens.

更新日期：2022-09-27

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