Ultrabright Renal-Clearable Cyanine-Protein Nanoprobes for High-Quality NIR-II Angiography and Lymphography,Nano Letters

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Ultrabright Renal-Clearable Cyanine-Protein Nanoprobes for High-Quality NIR-II Angiography and Lymphography
Nano Letters ( IF 9.6 ) Pub Date : 2022-09-27 , DOI: 10.1021/acs.nanolett.2c03311
Jiajun Xu ₁ , Tianyang Han ₁ , Yajun Wang ₁ , Feiran Zhang ₁ , Mengfei Li ₁ , Lang Bai ₁ , Xinyu Wang ₂ , Bin Sun _{1,

3} , Xin Wang ₃ , Jianshi Du ₂ , Kun Liu _{1,

3} , Junhu Zhang _{1,

3} , Shoujun Zhu _{1,

3}

Affiliation

The renal-clearable aspect of imaging agent with minimum toxicity issues and side effects is essential for clinical translation, yet clinical near-infrared-I/II (NIR-I/II) fluorophores with timely renal-clearance pathways are very limited. Herein, we rationally develop the cyanine-protein composite strategy through covalent bonding of β-lactoglobulin (β-LG) and chloride-cyanine dye to produce a brilliant and stable NIR-I/II fluorophore (e.g., β-LG@IR-780). The β-LG acts as a protecting shell with small molecular weight (18.4 kDa) and ultrasmall size (<5 nm), thus endowing the β-LG@IR-780 with excellent biocompatibility and renal excretion. Our β-LG@IR-780 probe enables noninvasive and precise NIR-II visualization of the physiological and pathological conditions of the vascular and lymphatic drainage system, facilitating intraoperative imaging-guided surgery and postoperative noninvasive monitoring. The minimum accumulation of our probes in the main organs improves the overall biosafety. This study provides a facile methodology for new-generation NIR-II fluorophores and largely improves the brightness and pharmacokinetics of small molecular dyes.

中文翻译：

用于高质量 NIR-II 血管造影和淋巴造影的超亮肾脏可清除花青蛋白纳米探针

具有最小毒性问题和副作用的显像剂的肾脏可清除方面对于临床转化至关重要，但具有及时肾脏清除途径的临床近红外-I/II (NIR-I/II) 荧光团非常有限。在此，我们通过 β-乳球蛋白 (β-LG) 和氯-菁染料的共价键合合理地开发了菁蛋白复合策略，以产生明亮且稳定的 NIR-I/II 荧光团（例如，β-LG@IR-780 ). β-LG作为保护壳具有小分子量（18.4 kDa）和超小尺寸（<5 nm），从而赋予β-LG@IR-780优异的生物相容性和肾脏排泄。我们的 β-LG@IR-780 探针能够对血管和淋巴引流系统的生理和病理状况进行无创和精确的 NIR-II 可视化，促进术中影像引导手术和术后无创监测。我们的探针在主要器官中的最小积累提高了整体生物安全性。本研究为新一代 NIR-II 荧光团提供了一种简便的方法，并大大提高了小分子染料的亮度和药代动力学。

更新日期：2022-09-27

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