Proper estimation of the energy consumption of tanker-based methods for the storage and transport of liquified CO₂ gas is a major concern in the techno-economic analysis and selection between the existing technologies within the carbon capture, utilization, and storage (CCUS) chain. In this study, the dynamic thermal modeling of a liquified CO₂ insulated-body tanker truck equipped with a refrigeration unit is presented to simulate the transient thermal performance of the tanker truck during a typical long-distance delivery mission. The main purpose of this research is to provide a more precise estimation of the heat leak quantity to the tanker, pressure build-up, and the refrigeration cooling demand and energy consumption. A two-way coupled set of unsteady-state differential equations are established on the participating elements included in the non-equilibrium thermal system of the tanker and refrigeration unit. The dynamic variations of ambient condition parameters, including the ambient temperature, solar radiation levels, degree of cloudiness, and wind velocity are incorporated into the model based on the annual average meteorological data in three modes of ambient conditions: the warmest week, a moderate week, and the coldest week of the year. The computational fluid dynamic (CFD) modeling of the liquid and gas phase CO₂ inside the vessel is also implemented in order to determine the required heat transfer coefficients used in the model. According to the modeling results, the average vessel inward heat leak changes from 11.08 W/m² in the warmest week of the year to 8.18 W/m² and 5.11 W/m² for a moderate and the coldest week, respectively, with 26.17 % and 53.88 % decrease. On the other hand, the refrigeration coefficient of performance (COP) is improved from 1.10 in the warmest week to 1.23 and 1.36 in a moderate and the coldest week, respectively, by 11.82 % and 23.63 % increase. As a measure of energy consumption of the refrigerated CO₂ tanker truck at different ambient conditions, the average compressor power consumption changes from 56 W/m³CO₂ in the warmest week of the year to 41 W/m³CO₂ and 28 W/m³CO₂ in a moderate and the coldest week, respectively, with 26.78 % and 50.00 % decrease. The novel modeling approach proposed in this study could also be used to study various design aspects of the refrigerated CO₂ transport and storage tankers.