Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates,Food Science & Nutrition

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Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2022-09-25 , DOI: 10.1002/fsn3.3058
Hui Huang ₁ , Jianwei Cen ₁ , Daqiao Yang _{1,

2} , Laihao Li ₁ , Chunsheng Li ₁ , Xianqing Yang ₁ , Yueqi Wang ₁ , Xiao Hu ₁ , Jinxu Wang ₃ , Qiuxing Cai _{3,

4}

Affiliation

Peptides from oysters have several bioactive functions. In this study, we identified antioxidant peptides from oysters (Crassostrea rivularis) and investigated their structure–function relationship. We used an 8 kDa molecular-weight (MW) cut-off membrane and semiprep reversed-phase liquid chromatography to collect five peptides (F1–F5) and identified the highest-abundance ion-peak sequences AWVDY (F1), MSFRFY(F2), EPLRY(F3), RKPPWPP(F4), and YAKRCFR(F5) having MWs of 652, 850, 676, 877, and 943 Da, respectively, using ultra-performance liquid chromatography-quadrupole/time-of-flight tandem mass spectrometry. These peptides exhibited high antioxidant activities, similar to butylated hydroxytoluene, reduced glutathione, and ascorbic acid. F5 demonstrated the highest scavenging activity for DPPH radicals (IC₅₀ = 21.75 μg/ml), hydroxyl radicals (IC₅₀ = 18.75 μg/ml), and superoxide radicals (IC₅₀ = 11.00 μg/ml), while F3 demonstrated the highest reducing power. Furthermore, F5 significantly protected Caco-2 cells from H₂O₂-induced oxidative damage. These results suggest that the antioxidant peptide F5 is a promising food additive that protects against oxidative damage.

中文翻译：

牡蛎（Crassostrea rivularis）蛋白酶解产物中抗氧化肽的分离和表征

牡蛎肽具有多种生物活性功能。在这项研究中，我们鉴定了来自牡蛎（ Crassostrea rivularis ）的抗氧化肽，并研究了它们的结构-功能关系。我们使用 8 kDa 分子量 (MW) 截留膜和半制备反相液相色谱收集了 5 种肽 (F1–F5)，并鉴定了丰度最高的离子峰序列 AWVDY (F1)、MSFRFY(F2)使用超高效液相色谱-四极杆/飞行时间串联质谱分析，EPLRY(F3)、RKPPWPP(F4) 和 YAKRCFR(F5) 的分子量分别为 652、850、676、877 和 943 Da 。这些肽表现出高抗氧化活性，类似于丁基羟基甲苯、还原型谷胱甘肽和抗坏血酸。 F5 对 DPPH 自由基 (IC ₅₀ = 21.75 μg/ml)、羟基自由基 (IC ₅₀ = 18.75 μg/ml) 和超氧自由基 (IC ₅₀ = 11.00 μg/ml) 表现出最高的清除活性，而 F3 表现出最高的还原活性力量。此外，F5 显着保护 Caco-2 细胞免受 H ₂ O ₂诱导的氧化损伤。这些结果表明，抗氧化肽 F5 是一种很有前途的食品添加剂，可以防止氧化损伤。

更新日期：2022-09-25

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