Nature Communications ( IF 14.7 ) Pub Date : 2022-09-24 , DOI: 10.1038/s41467-022-33341-6 Ani Baghdasaryan 1 , Feifei Wang 1 , Fuqiang Ren 1 , Zhuoran Ma 1 , Jiachen Li 1 , Xueting Zhou 2 , Lilit Grigoryan 3 , Chun Xu 1 , Hongjie Dai 1
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Sentinel lymph node imaging and biopsy is important to clinical assessment of cancer metastasis, and novel non-radioactive lymphographic tracers have been actively pursued over the years. Here, we develop gold molecular clusters (Au25) functionalized by phosphorylcholine (PC) ligands for NIR-II (1000–3000 nm) fluorescence imaging of draining lymph nodes in 4T1 murine breast cancer and CT26 colon cancer tumor mouse models. The Au-phosphorylcholine (Au-PC) probes exhibit ‘super-stealth’ behavior with little interactions with serum proteins, cells and tissues in vivo, which differs from the indocyanine green (ICG) dye. Subcutaneous injection of Au-PC allows lymph node mapping by NIR-II fluorescence imaging at an optimal time of ~ 0.5 − 1 hour postinjection followed by rapid renal clearance. Preclinical NIR-II fluorescence LN imaging with Au-PC affords high signal to background ratios and high safety and biocompatibility, promising for future clinical translation.
中文翻译:
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磷酸胆碱缀合的金分子簇可改善临床前癌症模型中淋巴结 NIR-II 荧光成像的信号
前哨淋巴结成像和活检对于癌症转移的临床评估很重要,多年来一直在积极探索新型非放射性淋巴结示踪剂。在这里,我们开发了金分子簇 (Au 25) 由磷酸胆碱 (PC) 配体功能化,用于 4T1 小鼠乳腺癌和 CT26 结肠癌肿瘤小鼠模型中引流淋巴结的 NIR-II (1000–3000 nm) 荧光成像。Au-磷酸胆碱 (Au-PC) 探针表现出“超级隐身”行为,与体内血清蛋白、细胞和组织几乎没有相互作用,这与吲哚菁绿 (ICG) 染料不同。皮下注射 Au-PC 允许在注射后约 0.5 - 1 小时的最佳时间通过 NIR-II 荧光成像进行淋巴结映射,然后进行快速肾脏清除。使用 Au-PC 的临床前 NIR-II 荧光 LN 成像可提供高信号背景比以及高安全性和生物相容性,有望用于未来的临床转化。