Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology,Analytical Chemistry

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Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-09-22 , DOI: 10.1021/acs.analchem.2c02036
Yaya Wang _{1,

2} , Ruiyi Zhao ₁ , Xinyue Zhu ₁ , Hong Gao ₁ , Can Gong ₁ , Xiaoyan Liu ₁ , Haixia Zhang ₁

Affiliation

Cell death plays a vital role in body development, maintenance of tissue function, and homeostasis. Accurate evaluation of cell death types is of great importance for pharmacological and pathological research. However, there is a lack of efficient fluorescent probes to discriminate various cell states. Here, we design and synthesize a novel activatable fluorescent probe PNE-Lyso to detect intracellular pH and hexosaminidases with two kinds of fluorescence signals. PNE-Lyso could distinguish dead cells from healthy cells based on a dual-color mode by targeting the lysosome and evaluating lysosomal hexosaminidase activity. Significantly, PNE-Lyso could also discriminate apoptotic and necrotic cells through visualizing lysosome morphology that is adjusted by the integrity of the lysosome membrane. Moreover, probe PNE-Lyso was successfully applied to investigate the drug-induced cell death process. To the best of our knowledge, this work is the first time cell death types have been distinguished based on a single fluorescent probe.

中文翻译：

通过可视化溶酶体形态用可激活荧光探针区分细胞死亡类型

细胞死亡在身体发育、组织功能维持和体内平衡中起着至关重要的作用。准确评估细胞死亡类型对于药理学和病理学研究具有重要意义。然而，缺乏有效的荧光探针来区分各种细胞状态。在这里，我们设计并合成了一种新型可激活荧光探针PNE-Lyso，以检测具有两种荧光信号的细胞内 pH 值和氨基己糖苷酶。PNE-Lyso可以通过靶向溶酶体并评估溶酶体己糖胺酶活性，基于双色模式区分死细胞和健康细胞。值得注意的是，PNE-Lyso还可以通过可视化由溶酶体膜的完整性调节的溶酶体形态来区分凋亡和坏死细胞。此外，探针PNE-Lyso成功应用于研究药物诱导的细胞死亡过程。据我们所知，这项工作是首次基于单个荧光探针区分细胞死亡类型。

更新日期：2022-09-22

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