通过可激活的近红外荧光探针诊断阿尔茨海默病和原位生物成像,Analytical Chemistry

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通过可激活的近红外荧光探针诊断阿尔茨海默病和原位生物成像
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-09-19 , DOI: 10.1021/acs.analchem.2c02627
Yuexia Yang ₁ , Liang Zhang ₂ , Jiemin Wang ₁ , Yuping Cao ₁ , Shuyan Li ₁ , Wenwu Qin ₁ , Yun Liu ₃

Affiliation

阿尔茨海默病（Alzheimer's disease，AD）是一种常见的神经退行性疾病，可使大脑神经系统迅速退化，并伴有一些特殊的认知和行为功能障碍。近期，丁酰胆碱酯酶（BChE）被报道为一种重要的酶，其活性可为AD的及时发现和诊断提供预测价值。因此，设计一种针对BChE的选择性和快速反应的检测工具是必不可少的。在这项研究中，我们开发了一种用于检测BChE活性的新型近红外荧光探针 ( Chy-1 )。出色的灵敏度、良好的生物相容性和 0.12 ng/mL 的检测下限 (LOD) 使该探针对BChE极具特异性，已成功用于生物成像。更重要的是，Chy-1不仅可以清楚地区分肿瘤和正常细胞，而且由于原位喷涂产生的荧光强度差异明显，可以在正常组织和癌组织之间形成清晰的界限。最重要的是，Chy-1还成功应用于跟踪 AD 小鼠模型中的BChE活性。基于这项研究，新型探针可能成为临床诊断和治疗肿瘤和神经退行性疾病的有力工具。

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Diagnosis of Alzheimer’s Disease and In Situ Biological Imaging via an Activatable Near-Infrared Fluorescence Probe

Alzheimer’s disease (AD) is a common neurodegenerative disease that makes the brain nervous system degenerate rapidly and is accompanied by some special cognitive and behavioral dysfunction. Recently, butyrylcholinesterase (BChE) was reported as an important enzyme, whose activity can provide predictive value for timely discovery and diagnosis of AD. Therefore, it is indispensable to design a detection tool for selective and rapid response toward BChE. In this study, we developed a novel near-infrared fluorescent probe (Chy-1) for the detection of BChE activity. An excellent sensitivity, good biocompatibility, and lower limit of detection (LOD) of 0.12 ng/mL made the probe extremely specific for BChE, which was successfully used in biological imaging. What is more, Chy-1 can not only clearly distinguish tumor from normal cells but also forms a clear boundary between the normal and cancer tissues due to the obvious difference in fluorescence intensity produced via in situ spraying. Most important of all, Chy-1 was also successfully applied to track the BChE activity in AD mouse models. Based on this research, the novel probe may be a powerful tool for clinical diagnosis and therapy of tumor and neurodegenerative diseases.

更新日期：2022-09-19

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