HClO and H₂S, as two kinds of crucial small biomolecules, are endowed various roles in biological organisms. The redox balance between HClO and H₂S is closely related to the physiological and pathological processes. Thus, it is significant to monitor the redox process between HClO and H₂S. Inspired by the advantages of ratiometric fluorescent probes, we firstly developed a reversible ratiometric fluorescent probe (BT-Se) for HClO and H₂S via combination of phenyl selenide as the response group and 2-(2′-hydroxyphenyl)benzothiazole dye as the fluorophore. The proposed probe BT-Se could detect HClO with well-separated dual emission (110 nm), fast response, good selectivity and sensitivity owing to the oxidation reaction of the Se atom induced by HClO. Moreover, only H₂S could effectively recover the fluorescence of the detection system to the original state via H₂S induced-reduction of selenoxide. Cell imaging studies demonstrated that the probe BT-Se was capable of ratiometric monitoring the changes of intracellular HClO/H₂S, which suggested that it has great potential for researching the biological functions of HClO and H₂S.