The characteristics of lab-scale ANAMMOX system for efficient nitrogen removal were tested by different inoculations (anaerobic granular sludge (AGS) + anammox sludge (AMS), R1; AGS alone, R2) in continuous flow reactors following start-up under starvation. TN removal rates were above 90 % in both reactors. Microbial community analysis showed that the nitrogen removal was the combined results of anammox and partial denitrification (PD), Higher microbial growth rate triggered by quorum-sensing (QS) caused R1 to secrete more extracellular polymeric substances (EPS) and promoted the growth of its particle size. However, the overproduction of EPS destroyed the particle structure with time, and led to a deterioration of operational stability in R1. R2 showed better nitrogen removal stability to high nitrogen loading rate (NLR). This was due to higher endogenous organics within R2, which inhibited cell aggregation and EPS overproduction. But undeniably, R1 had higher specific anammox activity (SAA) with higher anammox bacteria (AAOB) abundance (The abundance of Planctomycetota in R1 and R2 were 21.2 %, 7.71 %). The results suggest that the operational stability was influenced by a combination of particle properties and microbial metabolism, and that the inoculation of AGS is more conducive to efficient and economic nitrogen removal in wastewater treatment under starvation.

在饥饿状态下启动后，在连续流动反应器中通过不同的接种（厌氧颗粒污泥(AGS) + 厌氧污泥 (AMS)，R1；单独的 AGS，R2）测试了用于高效脱氮的实验室规模 ANAMMOX 系统的特性。两个反应器中的 TN 去除率均高于 90%。微生物群落分析表明，脱氮是厌氧氨氧化和部分反硝化(PD), 由群体感应 (QS) 触发的更高的微生物生长速率导致 R1 分泌更多的细胞外聚合物 (EPS) 并促进其粒径的增长。然而，EPS的过量生产会随着时间的推移破坏颗粒结构，并导致R1的运行稳定性恶化。R2对高氮负载率（NLR）表现出更好的脱氮稳定性。这是由于 R2 中较高的内源性有机物抑制了细胞聚集和 EPS 过度生产。但不可否认的是，R1 具有更高的厌氧氨氧化比活性 (SAA) 和较高的厌氧氨氧化细菌 (AAOB) 丰度（ Planctomycetota的丰度）在 R1 和 R2 中分别为 21.2 %、7.71 %)。结果表明，运行稳定性受颗粒特性和微生物代谢的综合影响，AGS的接种更有利于饥饿条件下废水处理中高效、经济的脱氮。