Aims: The present study aimed to assess the mode of action of previously reported anti-Mycobacterium tuberculosis benzo[d]imidazole-2-carboxamides against FtsZ along with their antibacterial potential. Materials & methods: The anti-mycobacterial action of benzo[d]imidazole-2-carboxamides against FtsZ was evaluated using inhibition of Bacillus subtilis 168, light scattering assay, circular dichroism spectroscopy, in silico molecular docking and molecular dynamics simulations. Results & conclusion: Three compounds (1k, 1o and 1e) were active against isoniazid-resistant strains. Four compounds (1h, 1i, 1o and 4h) showed >70% inhibition against B. subtilis 168. Compound 1o was the most potent inhibitor (91 ± 5% inhibition) of B. subtilis 168 FtsZ and perturbed its secondary structure. Molecular docking and molecular dynamics simulation of complexed 1o suggested M. tuberculosis FtsZ as a possible target for antitubercular activity.

中文翻译：

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具有抗结核活性的苯并[d]咪唑-2-甲酰胺衍生物对细菌 FtsZ 的抑制作用。

目的：本研究旨在评估先前报道的抗结核分枝杆菌苯并[d]咪唑-2-甲酰胺对 FtsZ 的作用方式及其抗菌潜力。材料和方法：使用抑制枯草芽孢杆菌 168、光散射测定、圆二色光谱、计算机分子对接和分子动力学模拟来评估苯并[d]咪唑-2-甲酰胺对 FtsZ 的抗分枝杆菌作用。结果与结论：三种化合物（1k、1o 和 1e）对异烟肼耐药菌株具有活性。四种化合物（1h、1i、1o 和 4h）对枯草芽孢杆菌 168 表现出 >70% 的抑制作用。化合物 1o 是枯草芽孢杆菌 168 FtsZ 最有效的抑制剂（抑制率为 91 ± 5%），并扰乱了其二级结构。