In this study, the main focus was on designing and synthesizing a novel magnetic nanobiocomposite and its application in hyperthermia cancer treatment. Regarding this aim, sodium alginate (SA) hydrogel with CaCl₂ cross-linker formed and modified by silk fibroin (SF) natural polymer and halloysite nanotubes (HNTs), followed by in situ Fe₃O₄ magnetic nanoparticles preparation. No important differences were detected in red blood cells (RBCs) hemolysis, confirming the high blood compatibility of the treated erythrocytes with this nanobiocomposite. Moreover, the synthesized SA hydrogel/SF/HNTs/Fe₃O₄ nanobiocomposite does not demonstrate toxicity toward HEK293T normal cell line after 48 and 72 h. The anticancer property of SA hydrogel/SF/HNTs/Fe₃O₄ nanobiocomposites against breast cancer cell lines was corroborated. The magnetic saturation of the mentioned magnetic nanobiocomposite was 15.96 emu g⁻¹. The specific absorption rate (SAR) was measured to be 22.3 W g⁻¹ by applying an alternating magnetic field (AMF). This novel nanobiocomposite could perform efficiently in the magnetic fluid hyperthermia process, according to the obtained results.

在这项研究中，主要重点是设计和合成一种新型磁性纳米生物复合材料及其在热疗癌症治疗中的应用。针对这一目标，海藻酸钠 (SA) 水凝胶与 CaCl ₂交联剂由丝素蛋白 (SF) 天然聚合物和埃洛石纳米管 (HNT) 形成和改性，然后原位制备 Fe ₃ O ₄磁性纳米粒子。在红细胞 (RBC) 溶血中没有检测到重要差异，证实了处理过的红细胞与这种纳米复合材料的高血液相容性。此外，合成的 SA 水凝胶/SF/HNTs/Fe ₃ O ₄纳米复合材料在 48 和 72 小时后未表现出对 HEK293T 正常细胞系的毒性。SA 水凝胶/SF/HNTs/Fe ₃ O ₄纳米复合材料对乳腺癌细胞系的抗癌特性得到证实。上述磁性纳米生物复合材料的磁饱和度为15.96 emu g ^-1。通过施加交变磁场(AMF)测得比吸收率(SAR)为22.3W g ^{-1 。}根据获得的结果，这种新型纳米生物复合材料可以在磁流体热疗过程中有效地发挥作用。