The important role of glycerophospholipid metabolism in the protective effects of polyphenol-enriched Tartary buckwheat extract against alcoholic liver disease,Food & Function

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The important role of glycerophospholipid metabolism in the protective effects of polyphenol-enriched Tartary buckwheat extract against alcoholic liver disease
Food & Function ( IF 5.1 ) Pub Date : 2022-09-14 , DOI: 10.1039/d2fo01518h
Peng Cao _{1,

2} , Yue Wu ₃ , Yaping Li ₄ , Liping Xiang _{1,

2} , Bingyu Cheng ₄ , Yixin Hu ₄ , Xin Jiang ₄ , Zhe Wang ₃ , Sanlan Wu _{1,

2} , Luqin Si ₄ , Qiang Yang ₃ , Jian Xu ₃ , Jiangeng Huang ₄

Affiliation

Alcoholic liver disease (ALD) is a mounting public health problem with significant medical, economic and social burdens. Tartary buckwheat (F. tataricum (L.) Gaertn, bitter buckwheat) is a kind of healthy and nutritious food, which has been demonstrated to protect against ALD, but the underlying mechanism has not been fully studied. Herein, we aimed to elucidate the beneficial effects of Tartary buckwheat extract (mainly composed of polyphenols including rutin, quercetin, kaempferol and kaempferol-3-O-rutinoside) in terms of lipid metabolism with the aid of lipidomic analysis. In our study, we employed C57BL/6J mice and a Lieber–DeCarli alcohol liquid diet to construct an ALD model and found that Tartary buckwheat extract was able to prevent ALD-induced histopathological lesions, liver injury and abnormal plasma lipid levels. These beneficial effects might be attributed to the regulation of energy metabolism-related genes (SIRT1, LKB1 and AMPK), lipid synthesis-related genes (ACC, SREBP1c and HMGR) and lipid oxidation-related genes (PPARα, CPT1 and CPT2). In addition, lipidomic profiling and KEGG pathway analysis showed that glycerophospholipid metabolism contributed the most to elucidating the regulatory mechanism of Tartary buckwheat extract. In specific, chronic ethanol intake reduced the level of phosphatidylcholines (PC) and increased the level of phosphatidylethanolamines (PE) in the liver, resulting in a decrease in the PC/PE ratio, which could be all significantly restored by Tartary buckwheat extract intervention, indicating that the Tartary buckwheat extract might regulate PC/PE homeostasis to exert its lipid-lowering effect. Overall, we demonstrated that Tartary buckwheat extract could prevent ALD by modulating hepatic glycerophospholipid metabolism, providing the theoretical basis for its further exploitation as a medical plant or nutritional food.

中文翻译：

甘油磷脂代谢在苦荞多酚提取物对酒精性肝病保护作用中的重要作用

酒精性肝病 (ALD) 是一个日益严重的公共卫生问题，具有重大的医疗、经济和社会负担。苦荞（F. tataricum (L.) Gaertn，苦荞）是一种健康营养的食品，已被证明可以预防ALD，但其潜在机制尚未得到充分研究。在此，我们旨在阐明苦荞提取物（主要由多酚组成，包括芦丁、槲皮素、山柰酚和山柰酚-3- O-rutinoside）在脂质组学分析的帮助下进行脂质代谢。在我们的研究中，我们使用 C57BL/6J 小鼠和 Lieber-DeCarli 酒精流质饮食构建 ALD 模型，发现苦荞提取物能够预防 ALD 诱导的组织病理学损伤、肝损伤和异常的血浆脂质水平。这些有益作用可能归因于能量代谢相关基因（SIRT1、LKB1 和 AMPK）、脂质合成相关基因（ACC、SREBP1c 和 HMGR）和脂质氧化相关基因（PPARα、CPT1 和 CPT2）的调节。此外，脂质组学分析和KEGG通路分析表明，甘油磷脂代谢对阐明苦荞提取物的调控机制贡献最大。具体来说，长期摄入乙醇会降低肝脏中的磷脂酰胆碱（PC）水平并增加磷脂酰乙醇胺（PE）的水平，从而导致 PC/PE 比值降低，这都可以通过苦荞提取物干预显着恢复，表明苦荞提取物可能通过调节 PC/PE 稳态发挥其降脂作用。总体而言，我们证明了苦荞提取物可以通过调节肝脏甘油磷脂代谢来预防ALD，为其作为药用植物或营养食品的进一步开发提供理论依据。表明苦荞提取物可能通过调节 PC/PE 稳态发挥其降脂作用。总体而言，我们证明了苦荞提取物可以通过调节肝脏甘油磷脂代谢来预防ALD，为其作为药用植物或营养食品的进一步开发提供理论依据。表明苦荞提取物可能通过调节 PC/PE 稳态发挥其降脂作用。总体而言，我们证明了苦荞提取物可以通过调节肝脏甘油磷脂代谢来预防ALD，为其作为药用植物或营养食品的进一步开发提供理论依据。

更新日期：2022-09-14

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